State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, PR China.
J Ethnopharmacol. 2012 Nov 21;144(2):255-60. doi: 10.1016/j.jep.2012.09.001. Epub 2012 Sep 11.
Traditionally, chicken embryo eggs are widely considered as an immunomodulatory health product in China and southeast Asia. Besides that, Compendium of Materia Medica, the most famous Chinese pharmacopoeia, has recorded that chicken embryo eggs contributed to the appetite increase and immune function enhancement, especially for the pregnant and aged.
Through assessing the immune efficacy of the chicken embryo extracts on immunocompetent cells, the immunomodulatory mechanism and components were explored preliminarily.
The raw material was subjected to in vitro gastric and pancreatic digestion successively, and then ligarine was added to separate the digested mixture to water-soluble fraction (WS) and lipid-soluble fraction (LS). Employing in vitro cell models, the immunomodulating activities of the extracts on immunosuppressed mouse were examined, at concentrations ranging from 10 to 500 μg/ml. The cell experiments consisted of the following experiments: spleen lymphocyte proliferation and IL-2 secretion, peritoneal macrophage phagocytosis and NO production. Before cell experiments, Kjeldal method and phenol-sulfuric acid method were employed respectively to determine the total protein and polysaccharide content of WS, and gas chromatography to analyze the fatty acid composition of LS.
LS significantly enhanced the spleen lymphocyte proliferation and IL-2 secretion, while peritoneal macrophage phagocytosis and NO production activity were elevated by WS. Both extracts exhibited an immunomodulatory effect with a dose-effect relationship.
The findings confirmed the chicken embryo eggs as a potent immunopotentiator on the immunosuppressed mouse, which would be useful for the fruitful application of the chicken embryo eggs to serve as a superior immunostimulatory tonic. Furthermore, the two extracts exhibited a different bioactivity tendency which makes it a significative work to screen the bioactive component for further study.
传统上，鸡胚蛋在中国和东南亚被广泛认为是一种免疫调节保健品。此外，《本草纲目》是中国最著名的药典，其中记载鸡胚蛋有助于增进食欲和增强免疫功能，尤其对孕妇和老年人有益。
通过评估鸡胚提取物对免疫活性细胞的免疫功效，初步探讨其免疫调节机制和成分。
原料先经过体外胃和胰腺消化，然后加入橄榄油分离消化混合物成水溶性部分（WS）和脂溶性部分（LS）。采用体外细胞模型，在 10-500μg/ml 的浓度范围内，检测提取物对免疫抑制小鼠的免疫调节活性。细胞实验包括以下实验：脾淋巴细胞增殖和 IL-2 分泌、腹腔巨噬细胞吞噬作用和 NO 产生。在细胞实验之前，分别采用 Kjeldal 法和苯酚-硫酸法测定 WS 的总蛋白和多糖含量，采用气相色谱法分析 LS 的脂肪酸组成。
LS 显著增强了脾淋巴细胞增殖和 IL-2 分泌，而 WS 则提高了腹腔巨噬细胞的吞噬作用和 NO 产生活性。两种提取物均表现出免疫调节作用，具有剂量效应关系。
研究结果证实鸡胚蛋对免疫抑制小鼠具有较强的免疫增强作用，这将有助于鸡胚蛋作为一种优良的免疫刺激补品得到广泛应用。此外，两种提取物表现出不同的生物活性趋势，这对于筛选生物活性成分进行进一步研究具有重要意义。
