College of Laboratory Medicine, Dalian Medical University, Dalian, 116044, Liaoning Province, China.
Department of Pathology and Forensic Medicine, Dalian Medical University, Dalian, 116044, Liaoning Province, China.
Sci Rep. 2019 Mar 11;9(1):4092. doi: 10.1038/s41598-019-40953-4.
Aging is spontaneous and inevitable processes that lead to changes in biological systems. The present paper was designed to investigate the anti-aging roles of chick embryo (CE) and nutrient mixture (NM) in aging rats. Aging was induced by administration of D-galactose (D-gal, 500 mg/kg/day for 90 days). CE and NM were administered to aging rats through different dose gavage once a day. Cognitive function assessment was performed using the Morris water maze test. At the end of experiment, serum and tissues were collected for immunity and antioxidation function. The organs and tissues were excised for histological study. The results demonstrated that CE plus NM was superior treatment to improve the histopathologic changes and reverse learning and memory impairment of the aging rats. CE plus NM also increased the spleen and thymus index as well as splenocyte proliferation, and reversed inflammatory cytokine levels. In addition, the biochemical index showed that CE plus NM could improve the antioxidant enzyme activity of the aging rats, decrease lipofuscin (LF) and glutamate content. CE plus NM also inhibited the activation of TLR4/NF-κB pathway stimulated by LPS in splenic B lymphocytes. Overall, these results seem to be implying that CE plus NM was used as potentially natural supplement or functional food for preventing aging.
衰老是导致生物系统发生变化的自发和不可避免的过程。本研究旨在探讨鸡胚（CE）和营养混合物（NM）对衰老大鼠的抗衰老作用。通过给予 D-半乳糖（D-gal，500mg/kg/天，共 90 天）诱导衰老。CE 和 NM 通过不同剂量的灌胃每天一次给予衰老大鼠。使用 Morris 水迷宫测试进行认知功能评估。实验结束时，收集血清和组织用于免疫和抗氧化功能。切除器官和组织进行组织学研究。结果表明，CE 加 NM 联合治疗可改善衰老大鼠的组织病理学变化，逆转学习和记忆障碍。CE 加 NM 还增加了脾脏和胸腺指数以及脾淋巴细胞增殖，并逆转了炎症细胞因子水平。此外，生化指标表明，CE 加 NM 可改善衰老大鼠的抗氧化酶活性，减少脂褐素（LF）和谷氨酸含量。CE 加 NM 还抑制了 LPS 刺激的脾 B 淋巴细胞中 TLR4/NF-κB 通路的激活。总体而言，这些结果似乎表明 CE 加 NM 可作为预防衰老的潜在天然补充剂或功能性食品。
