Kopachik W
Department of Zoology, Michigan State University, East Lansing 48824.
Exp Cell Res. 1990 Feb;186(2):394-7. doi: 10.1016/0014-4827(90)90323-3.
Protein synthesis by Polyspondylium violaceum in response to the chemoattractant glorin was analyzed by two-dimensional gel electrophoresis to determine if glorin can affect gene expression. When cells developing in a shaking suspension culture were given glorin, five proteins exhibited significantly increased and three proteins exhibited decreased incorporation of L-[35S]methionine. Glorin was active from 10-1000 nM, a concentration range within which cells are chemotactically active. The extent of the response was dependent on the concentration and the length of exposure to glorin. This evidence suggests that glorin may act in part to mediate changes in gene expression during development.
