Department of Ecology, Jinan University, Guangzhou 510632, China.
Environ Pollut. 2013 Jan;172:23-32. doi: 10.1016/j.envpol.2012.08.013. Epub 2012 Sep 13.
We tested antioxidant responses of the green microalga Pseudokirchneriella subcapitata exposed to different concentrations of the three antibiotics erythromycin (ETM), ciprofloxacin (CPF) and sulfamethoxazole (SMZ). Measurements included the level of lipid peroxidation, the total antioxidative capacity and three major antioxidant mechanisms: the ascorbate-glutathione cycle, the xanthophyll cycle and the enzyme activities of catalase (CAT), superoxide dismutase (SOD), guaiacol glutathione peroxidase (GPX) and glutathione-S-transferase (GST). Three antibiotics significantly affect the antioxidant system of P. subcapitata, but in different ways the alga was more tolerant to CPF and SMZ exposures than to ETM exposure. ETM caused reductions in AsA and GSH biosynthesis, ascorbate-glutathione cycle, xanthophylls cycle and antioxidant enzyme activities. The toxicity of CPF seems to be mainly overcome via induction of the ascorbate-glutathione cycle and CAT, SOD and GPX activities, while the toxicity of SMZ on the photosynthetic apparatus is predominantly reduced by the xanthophyll cycle and GST activity.
我们测试了三种抗生素（红霉素、环丙沙星和磺胺甲恶唑）暴露于不同浓度下对绿色微藻斜生栅藻的抗氧化反应。测量包括脂质过氧化水平、总抗氧化能力和三种主要的抗氧化机制：抗坏血酸-谷胱甘肽循环、叶黄素循环以及过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GPX) 和谷胱甘肽-S-转移酶 (GST) 的酶活性。三种抗生素显著影响斜生栅藻的抗氧化系统，但藻类对 CPF 和 SMZ 的暴露比 ETM 的暴露更具耐受性。ETM 导致 AsA 和 GSH 生物合成、抗坏血酸-谷胱甘肽循环、叶黄素循环和抗氧化酶活性降低。CPF 的毒性似乎主要通过诱导抗坏血酸-谷胱甘肽循环和 CAT、SOD 和 GPX 活性来克服，而 SMZ 对光合器的毒性主要通过叶黄素循环和 GST 活性来降低。
