Research Unit in Environmental and Evolutionary Biology (URBE), NARILIS, University of Namur (FUNDP), Namur, Belgium.
Fish Shellfish Immunol. 2012 Nov;33(5):1112-22. doi: 10.1016/j.fsi.2012.08.013. Epub 2012 Sep 12.
We evaluated the physiological and humoral immune responses of Eurasian perch submitted to 4-h hypoxia in either single or repeated way. Two generations (F1 and F5) were tested to study the potential changes in these responses with domestication. In both generations, single and repeated hypoxia resulted in hyperglycemia and spleen somatic index reduction. Glucose elevation and lysozyme activity decreased following repeated hypoxia. Complement hemolytic activity was unchanged regardless of hypoxic stress or domestication level. A 2D-DIGE proteomic analysis showed that some C3 components were positively modulated by single hypoxia while C3 up- and down-regulations and over-expression of transferrin were observed following repeated hypoxia. Domestication was associated with a low divergence in stress and immune responses to hypoxia but was accompanied by various changes in the abundance of serum proteins related to innate/specific immunity and acute phase response. Thus, it appeared that the humoral immune system was modulated following single and repeated hypoxia (independently of generational level) or during domestication and that Eurasian perch may display physiological acclimation to frequent hypoxic disturbances.
我们评估了欧洲鲈鱼在单次或重复缺氧 4 小时后的生理和体液免疫反应。为了研究驯化对这些反应的潜在变化，我们测试了两代（F1 和 F5）鱼。在两代鱼中，单次和重复缺氧都会导致高血糖和脾脏体指数降低。重复缺氧后，葡萄糖升高和溶菌酶活性下降。无论缺氧应激还是驯化水平如何，补体溶血活性都没有变化。2D-DIGE 蛋白质组学分析表明，一些 C3 成分在单次缺氧时被正向调节，而在重复缺氧时观察到 C3 的上调和下调以及转铁蛋白的过度表达。驯化与对缺氧应激和免疫反应的低差异相关，但伴随着与先天/特异性免疫和急性期反应相关的血清蛋白丰度的各种变化。因此，似乎体液免疫系统在单次和重复缺氧（独立于世代水平）或驯化期间被调节，而欧洲鲈鱼可能表现出对频繁缺氧干扰的生理适应。
