University of Namur (FUNDP), Research Unit in Environmental and Evolutionary Biology (URBE), Rue de Bruxelles, 61, B-5000 Namur, Belgium.
Fish Shellfish Immunol. 2011 Dec;31(6):1113-21. doi: 10.1016/j.fsi.2011.10.001. Epub 2011 Oct 13.
The current study aimed to evaluate the influence of domestication process on the stress response and subsequent immune modulation in Eurasian perch juveniles (Perca fluviatilis) submitted to chronic confinement. Briefly, F1 and F4 generations were confined into small-size tanks and sampled 7 and 55 days after stocking. Cortisol and glucose levels as well as lysozyme activity and immunoglobulin level were evaluated in the serum. Spleen Somatic Index and spleen ROS production were also measured. A proteomic analysis was performed on serum sampled on day 7. Finally, both generations were genetically characterized using a microsatellite approach. Globally, results revealed that chronic confinement did not elicit a typical stress response but resulted in a prolonged immune stimulation. Proteomic results suggested that domestication process influenced the immune status of perch submitted to chronic confinement as the F1 confined fish displayed lower abundance of C3 complement component, transferrin and Apolipoprotein E. Microsatellite data showed a strong genetic drift as well as reduced genetic diversity, allelic number and heterozygosity along with domestication process. The present work is the first to report that fish under domestication can develop an immune response, assessed by a combined approach, following recurrent challenges imposed by captive environment despite a reduced genetic variation.
本研究旨在评估驯化过程对慢性禁闭条件下欧亚鲈鱼幼鱼（Perca fluviatilis）应激反应和随后免疫调节的影响。简要地说，F1 和 F4 代被限制在小尺寸的水箱中，并在放养后 7 天和 55 天进行采样。血清中评估了皮质醇和葡萄糖水平以及溶菌酶活性和免疫球蛋白水平。还测量了脾脏体指数和脾脏 ROS 产生。在第 7 天采集血清进行蛋白质组分析。最后，使用微卫星方法对两代鱼进行遗传特征分析。总体而言，结果表明，慢性禁闭并未引发典型的应激反应，而是导致了长期的免疫刺激。蛋白质组学结果表明，驯化过程影响了慢性禁闭鲈鱼的免疫状态，因为 F1 禁闭鱼显示出较低的 C3 补体成分、转铁蛋白和载脂蛋白 E 的丰度。微卫星数据显示，随着驯化过程的进行，遗传漂变强烈，遗传多样性、等位基因数量和杂合度降低。本研究首次报道，尽管遗传变异减少，但经过圈养环境反复挑战后，处于驯化状态下的鱼类可以通过综合方法发展出免疫反应。
