大鼠肾上腺皮质线粒体的分离。
Isolation of rat adrenocortical mitochondria.
机构信息
Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.
出版信息
Biochem Biophys Res Commun. 2012 Oct 12;427(1):96-9. doi: 10.1016/j.bbrc.2012.09.014. Epub 2012 Sep 12.
Abstract
This report describes a relatively simple and reliable method for isolating adrenocortical mitochondria from rats in good, reasonably pure yield. These organelles, which heretofore have been unobtainable in isolated form from small laboratory animals, are now readily accessible. A high degree of mitochondrial purity is shown by the electron micrographs, as well as the structural integrity of each mitochondrion. That these organelles have retained their functional integrity is shown by their high respiratory control ratios. In general, the biochemical performance of these adrenal cortical mitochondria closely mirrors that of typical hepatic or cardiac mitochondria.
摘要
本报告描述了一种相对简单可靠的方法，可从大鼠中分离出良好、产量合理且纯度较高的肾上腺皮质线粒体。这些细胞器迄今为止无法从小型实验动物中以分离形式获得，现在已易于获得。电镜照片以及每个线粒体的结构完整性表明线粒体具有高度的纯度。这些细胞器保留了其功能完整性，其呼吸控制比很高。总的来说，这些肾上腺皮质线粒体的生化性能与典型的肝或心肌线粒体非常相似。
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