Department of Agricultural and Biological Engineering, Mississippi State University, Starkville, MS 39759, USA.
Appl Biochem Biotechnol. 2012 Nov;168(6):1568-83. doi: 10.1007/s12010-012-9879-1. Epub 2012 Sep 15.
The sugars present in bio-oil produced by fast pyrolysis can potentially be fermented by microbial organisms to produce cellulosic ethanol. This study shows the potential for microbial digestion of the aqueous fraction of bio-oil in an enrichment medium to consume glucose and produce ethanol. In addition to glucose, inhibitors such as furans and phenols are present in the bio-oil. A pure glucose enrichment medium of 20 g/l was used as a standard to compare with glucose and aqueous fraction mixtures for digestion. Thirty percent by volume of aqueous fraction in media was the maximum additive amount that could be consumed and converted to ethanol. Inhibitors were removed by extraction, activated carbon, air stripping, and microbial methods. After economic analysis, the cost of ethanol using an inexpensive fermentation medium in a large scale plant is approximately $14 per gallon.
由快速热解产生的生物油中存在的糖可以被微生物发酵，以生产纤维素乙醇。本研究表明，在富集培养基中，生物油的水相部分具有被微生物消化的潜力，从而消耗葡萄糖并产生乙醇。除了葡萄糖之外，生物油中还存在呋喃和酚类等抑制剂。本研究使用了 20g/L 的纯葡萄糖富集培养基作为标准，以比较葡萄糖和水相混合物的消化情况。在培养基中，水相的最大添加量为 30%，可以被消耗并转化为乙醇。通过萃取、活性炭、空气汽提和微生物方法去除抑制剂。经过经济分析，在大型工厂中使用廉价发酵培养基生产乙醇的成本约为每加仑 14 美元。
