Department of Sustainable Bioproducts, Mississippi State University, Mississippi State, MS 39762-9820, USA.
Department of Agricultural and Biological Engineering, Mississippi State University, Mississippi State, MS 39762-9632, USA.
Bioresour Technol. 2014 Jun;161:379-84. doi: 10.1016/j.biortech.2014.03.051. Epub 2014 Mar 20.
Utilization of 1,6-anhydro-β-d-glucopyranose (levoglucosan) present (11% w/v) in the water fraction of bio-oil for ethanol production will facilitate improvement in comprehensive utilization of total carbon in biomass. One of the major challenges for conversion of anhydrous sugars from the bio-oil water fraction to bio-ethanol is the presence of inhibitory compounds that slow or impede the microbial fermentation process. Removal of inhibitory compounds was first approached by n-butanol extraction. Optimal ratio of n-butanol and bio-oil water fraction was 1.8:1. Removal of dissolved n-butanol was completed by evaporation. Concentration of sugars in the bio-oil water fraction was performed by membrane filtration and freeze drying. Fermentability of the pyrolytic sugars was tested by fermentation of hydrolyzed sugars with Saccharomyces pastorianus lager yeast. The yield of ethanol produced from pyrolytic sugars in the bio-oil water fraction reached a maximum of 98% of the theoretical yield.
利用生物油水相中（11%w/v）存在的 1,6-脱水-β-d-吡喃葡萄糖（左旋葡聚糖），将有助于提高生物质中总碳的综合利用率。从生物油水相中无水糖转化为生物乙醇的主要挑战之一是存在抑制性化合物，这些化合物会减缓或阻碍微生物发酵过程。首先通过正丁醇萃取来去除抑制性化合物。正丁醇和生物油水相的最佳比例为 1.8:1。通过蒸发去除溶解的正丁醇。通过膜过滤和冷冻干燥浓缩生物油水相中的糖。用酿酒酵母巴氏酵母发酵水解糖来测试热解糖的发酵性。从生物油水相中热解糖产生的乙醇的产率达到了理论产率的 98%。
