血管舒张剂对灌注大鼠后肢中去甲肾上腺素和血管收缩介导的氧摄取有抑制作用，但对骨骼肌收缩诱导的氧摄取无抑制作用；对肌肉组织非寒战产热的影响。

Inhibition by vasodilators of noradrenaline and vasoconstrictor-mediated, but not skeletal muscle contraction-induced oxygen uptake in the perfused rat hindlimb; implications for non-shivering thermogenesis in muscle tissue.

Colquhoun E Q, Hettiarachchi M, Ye J M, Rattigan S, Clark M G

Department of Biochemistry, University of Tasmania, Hobart, Australia.

Gen Pharmacol. 1990;21(1):141-8. doi: 10.1016/0306-3623(90)90610-x.

Abstract

The effect of noradrenaline as well as of vasopressin and angiotensin II to increase oxygen uptake and perfusion pressure by the isolated perfused rat hindlimb were completely inhibited by the vasodilators, nitroprusside (0.5 mM), nifedipine (2.5 microM) and isoprenaline (50 nM). 2. Oxygen uptake due to sciatic nerve stimulation of skeletal muscle contraction was not inhibited by 0.5 mM nitroprusside but was found to increase further that produced by a maximum dose of either noradrenaline or angiotensin II. 3. Analysis of high energy phosphates in samples of freeze-clamped hindlimb muscle showed no difference before and after vasoconstrictor addition or with muscle sampled in vivo. 4. It is concluded that norepinephrine mediated increase in oxygen uptake by the perfused rat hindlimb results from its vasoconstrictor action.

摘要

去甲肾上腺素以及血管加压素和血管紧张素II对离体灌注大鼠后肢增加氧摄取和灌注压的作用，被血管扩张剂硝普钠（0.5 mM）、硝苯地平（2.5 microM）和异丙肾上腺素（50 nM）完全抑制。2. 坐骨神经刺激骨骼肌收缩引起的氧摄取不受0.5 mM硝普钠抑制，且发现其比最大剂量的去甲肾上腺素或血管紧张素II所产生的氧摄取进一步增加。3. 对冷冻钳夹的后肢肌肉样本中的高能磷酸盐分析表明，添加血管收缩剂前后或与体内采样的肌肉相比没有差异。4. 得出的结论是，去甲肾上腺素介导的灌注大鼠后肢氧摄取增加是其血管收缩作用的结果。