Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5S 3E1, Canada.
Science. 2012 Sep 14;337(6100):1353-6. doi: 10.1126/science.1224339.
The dimorphic switch from a single-cell budding yeast to a filamentous form enables Saccharomyces cerevisiae to forage for nutrients and the opportunistic pathogen Candida albicans to invade human tissues and evade the immune system. We constructed a genome-wide set of targeted deletion alleles and introduced them into a filamentous S. cerevisiae strain, Σ1278b. We identified genes involved in morphologically distinct forms of filamentation: haploid invasive growth, biofilm formation, and diploid pseudohyphal growth. Unique genes appear to underlie each program, but we also found core genes with general roles in filamentous growth, including MFG1 (YDL233w), whose product binds two morphogenetic transcription factors, Flo8 and Mss11, and functions as a critical transcriptional regulator of filamentous growth in both S. cerevisiae and C. albicans.
从单细胞出芽酵母到丝状形态的二相转变使酿酒酵母能够寻找营养物质，而机会性病原体白色念珠菌能够侵袭人体组织并逃避免疫系统。我们构建了一个全基因组靶向缺失基因座的集合，并将其引入丝状酿酒酵母菌株 Σ1278b 中。我们鉴定了参与形态上不同丝状生长形式的基因：单倍体侵袭性生长、生物膜形成和二倍体假菌丝生长。每个程序似乎都有独特的基因，但我们也发现了在丝状生长中具有普遍作用的核心基因，包括 MFG1（YDL233w），其产物结合两种形态发生转录因子 Flo8 和 Mss11，并作为酿酒酵母和白色念珠菌丝状生长的关键转录调节剂发挥作用。
