酿酒酵母S288C在FLO8基因中存在突变，FLO8基因是丝状生长所必需的基因。
Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for filamentous growth.
作者信息
Liu H, Styles C A, Fink G R
机构信息
Department of Biological Chemistry, College of Medicine, University of California, Irvine 92717, USA.
出版信息
Genetics. 1996 Nov;144(3):967-78. doi: 10.1093/genetics/144.3.967.
Abstract
Diploid strains of baker's yeast Saccharomyces cerevisiae can grow in a cellular yeast form or in filaments called pseudohyphae. This dimorphic transition from yeast to pseudohyphae is induced by starvation for nitrogen. Not all laboratory strains are capable of this dimorphic switch; many grow only in the yeast form and fail to form pseudohyphae when starved for nitrogen. Analysis of the standard laboratory strain S288C shows that this defect in dimorphism results from a nonsense mutation in the FLO8 gene. This defect in FLO8 blocks pseudohyphal growth in diploids, haploid invasive growth, and flocculation. Since feral strains of S. cerevisiae are dimorphic and have a functional FLO8 gene, we suggest that the flo8 mutation was selected during laboratory cultivation.
摘要
面包酵母酿酒酵母的二倍体菌株可以以细胞酵母形式生长，也可以以称为假菌丝的丝状形式生长。从酵母到假菌丝的这种双态转变是由氮饥饿诱导的。并非所有实验室菌株都能进行这种双态转换；许多菌株仅以酵母形式生长，在氮饥饿时无法形成假菌丝。对标准实验室菌株S288C的分析表明，这种双态性缺陷是由FLO8基因中的无义突变导致的。FLO8的这种缺陷会阻止二倍体中的假菌丝生长、单倍体侵袭性生长和絮凝。由于酿酒酵母的野生菌株具有双态性且具有功能性FLO8基因，我们认为flo8突变是在实验室培养过程中被选择出来的。
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本文引用的文献
1
Ras2 signals via the Cdc42/Ste20/mitogen-activated protein kinase module to induce filamentous growth in Saccharomyces cerevisiae.Ras2通过Cdc42/Ste20/丝裂原活化蛋白激酶模块发出信号，以诱导酿酒酵母中的丝状生长。
Proc Natl Acad Sci U S A. 1996 May 28;93(11):5352-6. doi: 10.1073/pnas.93.11.5352.
2
Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6 kilobase pairs.酿酒酵母六条最小染色体的物理图谱，分辨率为2.6千碱基对。
Genetics. 1993 May;134(1):81-150. doi: 10.1093/genetics/134.1.81.
3
Sequence of the open reading frame of the FLO1 gene from Saccharomyces cerevisiae.来自酿酒酵母的FLO1基因开放阅读框的序列。
Yeast. 1993 Apr;9(4):423-7. doi: 10.1002/yea.320090413.
4
A general suppressor of RNA polymerase I, II and III mutations in Saccharomyces cerevisiae.酿酒酵母中RNA聚合酶I、II和III突变的一般抑制因子。
Mol Gen Genet. 1993 May;239(1-2):169-76. doi: 10.1007/BF00281615.
5
Regulation of dimorphism in Saccharomyces cerevisiae: involvement of the novel protein kinase homolog Elm1p and protein phosphatase 2A.酿酒酵母中双态性的调控：新型蛋白激酶同源物Elm1p和蛋白磷酸酶2A的作用。
Mol Cell Biol. 1993 Sep;13(9):5567-81. doi: 10.1128/mcb.13.9.5567-5581.1993.
6
A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast.一种与酵母中的RNA聚合酶II CTD和TATA结合蛋白相关的多亚基复合物。
Cell. 1993 Jul 2;73(7):1361-75. doi: 10.1016/0092-8674(93)90362-t.
7
Reversible pseudohyphal growth in haploid Saccharomyces cerevisiae is an aerobic process.单倍体酿酒酵母中的可逆假菌丝生长是一个需氧过程。
Curr Genet. 1993 May-Jun;23(5-6):388-91. doi: 10.1007/BF00312623.
8
Elements of the yeast pheromone response pathway required for filamentous growth of diploids.二倍体丝状生长所需的酵母信息素反应途径的元件。
Science. 1993 Dec 10;262(5140):1741-4. doi: 10.1126/science.8259520.
9
Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development.通过过表达PHD1诱导假菌丝生长，PHD1是酿酒酵母中一个与真菌发育转录调节因子相关的基因。
Mol Cell Biol. 1994 Mar;14(3):2100-12. doi: 10.1128/mcb.14.3.2100-2112.1994.
10
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