van der Waal Suzette V, Jiang Lei-Meng, de Soet Johannes J, van der Sluis Lucas W M, Wesselink Paul R, Crielaard Wim
Department of Conservative & Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, the Netherlands.
Eur J Oral Sci. 2012 Oct;120(5):452-7. doi: 10.1111/j.1600-0722.2012.00982.x. Epub 2012 Aug 10.
Incomplete disinfection of the root canal system is a major cause of post-treatment disease. This study aimed to investigate the disinfecting property of organic acid salts and sodium chloride (NaCl), in a double-hurdle strategy, on Enterococcus faecalis biofilms. First of all, the high-throughput resazurin metabolism assay (RMA) was used to test a range of organic acid salts. Then, to gain more insight into the efficacy of sorbate salt solutions, 48-h E. faecalis biofilms were evaluated in colony-forming unit (CFU) assays. Chlorhexidine (CHX) and calcium hydroxide [Ca(OH)(2) ] were tested in parallel as controls. Sorbate salt produced the largest and most significant reduction of fluorescence intensity in the RMA assay. Neither NaCl nor potassium sorbate (KS) alone induced a clinically relevant reduction of CFU counts after 1 h. Surprisingly, the combination of the two in a single solution had a synergistic effect on the inactivation of E. faecalis. Potassium sorbate amplified the efficacy of NaCl. Of the salts tested, NaCl with KS eradicated E. faecalis biofilms within 1 h. This study showed that the double-hurdle strategy indeed leads to synergistic efficacy and is a possible next step in the complete disinfection of endodontic infections.
根管系统消毒不彻底是治疗后疾病的主要原因。本研究旨在探讨有机酸盐和氯化钠（NaCl）在双障碍策略中对粪肠球菌生物膜的消毒特性。首先，采用高通量刃天青代谢试验（RMA）来测试一系列有机酸盐。然后，为了更深入了解山梨酸盐溶液的效果，在菌落形成单位（CFU）试验中对48小时的粪肠球菌生物膜进行评估。同时测试洗必泰（CHX）和氢氧化钙[Ca(OH)₂]作为对照。在RMA试验中，山梨酸盐使荧光强度降低幅度最大且最显著。单独使用NaCl或山梨酸钾（KS）1小时后均未引起临床上相关的CFU计数减少。令人惊讶的是，两者在单一溶液中的组合对粪肠球菌的灭活具有协同作用。山梨酸钾增强了NaCl的效果。在所测试的盐中，NaCl与KS的组合在1小时内根除了粪肠球菌生物膜。本研究表明，双障碍策略确实能产生协同效果，是牙髓感染彻底消毒可能的下一步措施。
