Oztan Meltem Dartar, Kiyan Mehmet, Gerçeker Devran
Faculty of Dentistry, Department of Endodontics, Ankara University, Ankara, Turkey.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Sep;102(3):410-6. doi: 10.1016/j.tripleo.2005.10.073. Epub 2006 May 19.
The objective of this study was to determine the in vitro susceptibility of Enterococcus faecalis and the most prevalent Candida species as therapy-resistant microorganisms to gutta-percha points containing root canal medications.
Gutta-percha points containing calcium hydroxide (Calcium Hydroxide Plus Points), chlorhexidine diacetate (Activ Points), or calcium hydroxide-chlorhexidine combinations (CHX/Ca Combi Points) were tested for their ability to inhibit growth of pure cultures of Candida albicans, Candida glabrata, Candida parapsilosis, Candida krusei, Candida tropicalis, Saccharomyces cerevisiae, and Enterococcus faecalis. Approximately 2 x 10(7) microorganisms per assay were suspended in diluted human serum and co-incubated with the gutta-percha points placed in Eppendorf tubes in an incubator for up to 2 weeks. A tube was removed at 1, 2, 3, 4, 7, and 14 days, and then opened and microorganism suspensions were serially diluted in a sterile 0.9% NaCl solution. Aliquots of the dilution steps were streaked onto solid medium. After incubating the plates in an incubator at 37 +/- 1 degrees C for 48 hours, CFU numbers per milliliter of suspension were calculated.
Calcium Hydroxide Plus Points or Activ Points did not exhibit sufficient antimicrobial or anticandidal activity for Enterococcus faecalis, Candida albicans, C. glabrata, C. parapsilosis, C. krusei, or C. tropicalis within 14 days. Only Saccharomyces cerevisiae was susceptible to the calcium hydroxide or chlorhexidine diacetate containing gutta percha points. CHX/Ca Combi Points killed C. albicans, C. glabrata, C. krusei, C. tropicalis, and S. cerevisiae completely. However, E. faecalis and C. parapsilosis were resistant to CHX/Ca Combi points within 14 days.
The results show the gutta percha points containing a mixture of calcium hydroxide and chlorhexidine diacetate have efficacies superior to calcium hydroxide or chlorhexidine diacetate alone against some microorganisms except E. faecalis and C. parapsilosis.
本研究的目的是确定粪肠球菌和最常见的念珠菌属作为耐药微生物对含根管药物的牙胶尖的体外敏感性。
对含氢氧化钙的牙胶尖（氢氧化钙加牙胶尖）、双醋酸氯己定的牙胶尖（活性牙胶尖）或氢氧化钙 - 氯己定组合的牙胶尖（CHX/Ca组合牙胶尖）进行测试，以评估它们抑制白色念珠菌、光滑念珠菌、近平滑念珠菌、克柔念珠菌、热带念珠菌、酿酒酵母和粪肠球菌纯培养物生长的能力。每次试验中约2×10⁷个微生物悬浮于稀释的人血清中，并与置于Eppendorf管中的牙胶尖在培养箱中共同孵育长达2周。在第1、2、3、4、7和14天取出一支试管，然后打开，将微生物悬液在无菌0.9%氯化钠溶液中进行系列稀释。取稀释步骤的等分试样划线接种于固体培养基上。将平板在37±1℃培养箱中孵育48小时后，计算每毫升悬液中的菌落形成单位数。
氢氧化钙加牙胶尖或活性牙胶尖在14天内对粪肠球菌、白色念珠菌、光滑念珠菌、近平滑念珠菌、克柔念珠菌或热带念珠菌未表现出足够的抗菌或抗念珠菌活性。只有酿酒酵母对含氢氧化钙或双醋酸氯己定的牙胶尖敏感。CHX/Ca组合牙胶尖可完全杀灭白色念珠菌、光滑念珠菌、克柔念珠菌、热带念珠菌和酿酒酵母。然而，粪肠球菌和近平滑念珠菌在14天内对CHX/Ca组合牙胶尖耐药。
结果表明，含氢氧化钙和双醋酸氯己定混合物的牙胶尖对除粪肠球菌和近平滑念珠菌外的某些微生物的疗效优于单独的氢氧化钙或双醋酸氯己定。
