Department of Medical Biotechnology and Translational Medicine, University of Milan , Via Fratelli Cervi 93, 20090 Segrate (Milan), Italy.
J Agric Food Chem. 2012 Oct 10;60(40):10103-10. doi: 10.1021/jf3027414. Epub 2012 Oct 1.
Ceramides are presented as nutraceutical compounds for protection of colon carcinoma and as important cosmetic preparation components, increasing absorption through the skin. Therefore, the ceramide (Cer) content of Moro blood oranges was determined by mass spectrometry. A total of 114 Cer species were identified: ∼160 mg in the peels and ∼140 mg in the pulp per kilogram of oranges, expressed as "milligram equivalents of d18:1,17:0 Cer". The predominant ceramides contained 4-hydroxy-8-sphingenine (t18:1(Δ8)) and 4-hydroxysphinganine (t18:0) as long-chain bases (LCBs) and fatty acids (FAs) with different structures. In the pulp, t18:1(Δ8)- and t18:0-containing Cer species comprised 50.5 and 33.5% of the total, respectively, 11.5 and 3.5% non-hydroxylated FAs, respectively, 32.0 and 21.0% α-hydroxylated FAs, respectively, and 7.0 and 9.0% α,β-hydroxylated FAs, respectively. In the peels, t18:1(Δ8)- and t18:0-containing species comprised 49.5 and 34.5% of the total, respectively, 16.0 and 1.5% non-hydroxylated FAs, respectively, 31.5 and 29.0% α-hydroxylated FAs, respectively, and 2.0 and 4.0% α,β-hydroxylated FAs, respectively.
神经酰胺被认为是保护结肠癌的营养化合物，也是重要的化妆品制备成分，可增加皮肤的吸收率。因此，通过质谱法测定了摩洛血橙中的神经酰胺（Cer）含量。共鉴定出 114 种 Cer 种：每公斤橙子的果皮中约有 160 毫克，果肉中约有 140 毫克，以“d18:1，17:0 Cer 的毫克当量”表示。主要的神经酰胺含有 4-羟基-8-神经鞘氨醇（t18:1（Δ8））和 4-羟基神经鞘氨醇（t18:0）作为长链碱基（LCB）和具有不同结构的脂肪酸（FA）。在果肉中，t18:1（Δ8）-和 t18:0 含量的 Cer 种分别占总 Cer 的 50.5%和 33.5%，非羟基化 FA 分别占 11.5%和 3.5%，α-羟基化 FA 分别占 32.0%和 21.0%，α，β-羟基化 FA 分别占 7.0%和 9.0%。在果皮中，t18:1（Δ8）-和 t18:0 含量的 Cer 种分别占总 Cer 的 49.5%和 34.5%，非羟基化 FA 分别占 16.0%和 1.5%，α-羟基化 FA 分别占 31.5%和 29.0%，α，β-羟基化 FA 分别占 2.0%和 4.0%。
