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超高效液相色谱-电喷雾离子化-四极杆飞行时间质谱法推测辣椒红中神经酰胺的存在。

Speculation of Sphingolipids in Capsanthin by Ultra-Performance Liquid Chromatography Coupled with Electrospray Ionization-Quadrupole-Time-of-Flight Mass Spectrometry.

机构信息

Chenguang Biological Technology Group Co., Ltd., Handan 057250, China.

Chenguang Biotech Group Handan Co., Ltd., Handan 056000, China.

出版信息

Molecules. 2023 Jan 19;28(3):1010. doi: 10.3390/molecules28031010.

DOI:10.3390/molecules28031010
PMID:36770678
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9920690/
Abstract

Sphingolipids are constituents of cellular membranes and play important roles in cells. As nutraceutical compounds in foods, sphingolipids have been proven to be critical for human health. Therefore, the sphingolipids content of capsanthin was established based on ultra-performance liquid chromatography coupled with electrospray ionization-quadrupole-time-of-flight mass spectrometry. A total number of 40 sphingolipids were successfully identified, including 20 Glucosylceramides and 20 Ceramides. The predominant GlcCers contain 4-hydroxy-8-sphingenine t18:1 () with different structures of α-OH fatty acids. For the Cers, the main long-chain bases are 4-hydroxy-8-sphingenine t18:1 () and 4-hydroxysphingenine (t18:0) with different structures of α-OH or α, β-di (OH) fatty acids.

摘要

鞘脂类是细胞膜的组成部分,在细胞中发挥着重要作用。作为食品中的营养化合物,鞘脂类已被证明对人类健康至关重要。因此,基于超高效液相色谱-电喷雾电离-四极杆-飞行时间质谱联用技术,建立了辣椒红素中的鞘脂类含量。成功鉴定了 40 种鞘脂类物质,包括 20 种葡萄糖神经酰胺和 20 种神经酰胺。主要的 GlcCers 含有 4-羟基-8-神经鞘氨醇 t18:1(),其α-OH 脂肪酸结构不同。对于 Cers,主要的长链碱基是 4-羟基-8-神经鞘氨醇 t18:1()和 4-羟基神经鞘氨醇(t18:0),其α-OH 或α、β-二(OH)脂肪酸的结构不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26b/9920690/ba45cfe0b35f/molecules-28-01010-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26b/9920690/d6414f79e084/molecules-28-01010-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26b/9920690/7ba208a04872/molecules-28-01010-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26b/9920690/4f6385fef55c/molecules-28-01010-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26b/9920690/ba45cfe0b35f/molecules-28-01010-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26b/9920690/d6414f79e084/molecules-28-01010-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26b/9920690/7ba208a04872/molecules-28-01010-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26b/9920690/4f6385fef55c/molecules-28-01010-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f26b/9920690/ba45cfe0b35f/molecules-28-01010-g004.jpg

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