Ruiz Carlos F, Ray Candis L, Cook Melissa, Grace Mark A, Bullard Stephen A
Aquatic Parasitology Laboratory, Department of Fisheries and Allied Aquacultures, College of Agriculture, Auburn University, 203 Swingle Hall, Auburn, Alabama 36849, USA.
J Parasitol. 2013 Apr;99(2):318-26. doi: 10.1645/GE-3249.1. Epub 2012 Sep 17.
Eggs and larvae of Huffmanela oleumimica n. sp. infect red snapper, Lutjanus campechanus (Poey, 1860), were collected from the Texas-Louisiana Shelf (28°16'36.58″N, 93°03'51.08″W) and are herein described using light and scanning electron microscopy. Eggs in skin comprised fields (1-5 × 1-12 mm; 250 eggs/mm(2)) of variously oriented eggs deposited in dense patches or in scribble-like tracks. Eggs had clear (larvae indistinct, principally vitelline material), amber (developing larvae present) or brown (fully developed larvae present; little, or no, vitelline material) shells and measured 46-54 μm (x = 50; SD ± 1.6; n = 213) long, 23-33 (27 ± 1.4; 213) wide, 2-3 (3 ± 0.5; 213) in eggshell thickness, 18-25 (21 ± 1.1; 213) in vitelline mass width, and 36-42 (39 ± 1.1; 213) in vitelline mass length with protruding polar plugs 5-9 (7 ± 0.6; 213) long and 5-8 (6 ± 0.5; 213) wide. Fully developed larvae were 160-201 (176 ± 7.9) long and 7-8 (7 ± 0.5) wide, had transverse cuticular ridges, and were emerging from some eggs within and beneath epidermis. The new species differs from its congeners by having eggs <65 μm in total length and that have a brown eggshell when fully developed, an envelope throughout development, and irregularly-dispersed eggshell spines plus a larva >110 μm long with transverse cuticular ridges. The eggs lack a spindle-shaped envelope, polar filaments, and eggshell ridges. This is the first report of a species of Huffmanela from a snapper (Lutjanidae) or from the Gulf of Mexico. A table of egg and larval characteristics, hosts, and localities for Huffmanela spp. is provided.
从得克萨斯州 - 路易斯安那州大陆架（北纬28°16'36.58″，西经93°03'51.08″）采集到感染红鲷（Lutjanus campechanus，Poey，1860）的油栖赫夫曼线虫（Huffmanela oleumimica）新种的卵和幼虫，并在此使用光学显微镜和扫描电子显微镜进行描述。皮肤中的卵形成不同方向的卵区（1 - 5×1 - 12毫米；每平方毫米250个卵），这些卵密集分布成斑块状或呈潦草状轨迹。卵有透明的（幼虫不明显，主要是卵黄物质）、琥珀色的（有发育中的幼虫）或棕色的（有完全发育的幼虫；卵黄物质很少或没有）壳，长46 - 54微米（平均值 = 50；标准差±1.6；n = 213），宽23 - 33（27±1.4；213）微米，卵壳厚度2 - 3（3±0.5；213）微米，卵黄团宽度18 - 25（21±1.1；213）微米，卵黄团长度36 - 42（39±1.1；213）微米，突出的极栓长5 - 9（7±0.6；213）微米，宽5 - 8（6±0.5；213）微米。完全发育的幼虫长160 - 201（176±7.9）微米，宽7 - 8（7±0.5）微米，有横向表皮嵴，并且正从表皮内和表皮下的一些卵中孵出。该新物种与其同属物种的区别在于，其卵全长小于65微米，完全发育时卵壳为棕色，发育过程中有包膜，卵壳刺不规则分布，幼虫长大于110微米且有横向表皮嵴。这些卵没有纺锤形包膜、极丝和卵壳嵴。这是关于来自鲷科鱼类（Lutjanidae）或墨西哥湾的赫夫曼线虫属物种的首次报道。提供了赫夫曼线虫属物种的卵和幼虫特征、宿主及产地的表格。
