Department of Physiology, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of Korea.
Neurosci Lett. 2012 Oct 31;529(1):97-101. doi: 10.1016/j.neulet.2012.08.075. Epub 2012 Sep 7.
In this study, we investigated the effects of saikosaponin A (SSA), a major compound of Bupleurum falcatum L., on morphine self-administration behavior. Male Sprague-Dawley rats were trained to self-administer intravenous morphine (0.1mg/kg per injection over 5s) during daily 1-h sessions under a fixed-ratio 1 schedule. Rats were pretreated with SSA (0.25, 0.5, 1.0mg/kg) by intraperitoneal injection 30 min prior to the start of the test session. Results demonstrated that pretreatment with SSA reduced morphine-maintained responding dose-dependently. Additionally, SSA inhibition of morphine-reinforced behavior was blocked by the selective GABA(B) receptor antagonist (2S)(+)-5,5-dimethyl-2-morpholineacetic acid (SCH 50911), but not the selective GABA(A) receptor antagonist bicuculline. Together, these results suggest that SSA may effectively suppress morphine-reinforced behavior by activating GABA(B) receptors.
在这项研究中，我们研究了柴胡皂苷 A（SSA）对吗啡自我给药行为的影响。雄性 Sprague-Dawley 大鼠在固定比率 1 方案下，每天进行 1 小时的静脉注射吗啡（每次 0.1mg/kg，持续 5s）训练，以自我给药。大鼠在测试开始前 30 分钟通过腹腔注射预先用 SSA（0.25、0.5、1.0mg/kg）预处理。结果表明，SSA 预处理剂量依赖性地减少了吗啡维持的反应。此外，选择性 GABA(B) 受体拮抗剂（2S）（+）-5,5-二甲基-2-吗啉乙酸（SCH 50911）阻断了 SSA 对吗啡强化行为的抑制作用，但选择性 GABA(A) 受体拮抗剂荷包牡丹碱则没有。总之，这些结果表明 SSA 可能通过激活 GABA(B) 受体有效抑制吗啡强化行为。
