Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, People's Republic of China.
Neurosci Lett. 2012 Oct 11;527(2):115-20. doi: 10.1016/j.neulet.2012.08.082. Epub 2012 Sep 7.
Quercetin is one of flavonoids with cyto-protective activities. It has been demonstrated that quercetin inhibits oxidative stress in some animal models and specific cells, but the particular mechanism is known a little. In the present study, we found that quercetin could decrease the expression of Krüppel-like factor 4 (KLF4) induced by hydrogen peroxide (H(2)O(2)) in human neuroblastoma SH-SY5Y cells, further increase the expression ratio of bcl-2 to bax, which were apoptotic-related target genes of KLF4, thus alleviate the apoptotic rate and caspase-3 enzyme activity of SH-5YSY cells; the overexpression or inhibition of KLF4 demonstrated the mediated role of KLF4 for the protective effect of quercetin on cell damage induced by H(2)O(2). All results suggest a potential molecular mechanism of quercetin protecting against the oxidative damage, which may be applied in the treatment of oxidative related diseases, such as neurodegeneration diseases.
槲皮素是具有细胞保护活性的类黄酮之一。已经证明，槲皮素可以抑制某些动物模型和特定细胞中的氧化应激，但具体机制知之甚少。在本研究中，我们发现槲皮素可以降低人神经母细胞瘤 SH-SY5Y 细胞中过氧化氢（H 2 O 2 ）诱导的 Krüppel 样因子 4（KLF4）的表达，进一步增加凋亡相关靶基因 bcl-2 与 bax 的表达比例，从而减轻 SH-SY5Y 细胞的凋亡率和 caspase-3 酶活性；KLF4 的过表达或抑制证明了 KLF4 在槲皮素对 H 2 O 2 诱导的细胞损伤的保护作用中的介导作用。所有结果表明槲皮素对抗氧化损伤的潜在分子机制，可应用于治疗与氧化相关的疾病，如神经退行性疾病。
