Wang Yuhuan, Zhang Min, Yan Lili, Ding Shimei, Xie Xuan
Department of Endocrinology, Second Hospital Affiliated to Xi'an Jiaotong University College of Medicine, Xi'an 710004, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Sep;32(9):1373-6.
To investigate serum APPL1 level in patients with newly diagnosed type 2 diabetes mellitus (T2DM) and analyze its correlation with body mass index (BMI), waist-to-hip ratio (WHR), blood pressure, fasting plasma glucose, fasting blood insulin, HbA1c, and insulin resistance (HOMA-IR).
Serum APPL1 levels were determined using ELISA in 22 normal control subjects and 63 patients with newly diagnosed T2DM, and HOMA-IR of the subjects was calculated using the HOMA model.
The diabetic patients had significantly elevated levels of serum APPL1 compared with the control subjects (85.71∓27.39 vs 64.52∓16.28 pg/ml, P<0.01), with also significantly increased BMI, WHR, SBP, FINS, LgHOMA-IR (P<0.01) and LDL-C (P<0.05) but lowered HDL-C (P<0.01). Fasting serum APPL1 levels were positively correlated with FPG, FINS, and HOMA-IR (r=0.215, 0.297, 0.334, P=0.014, 0.006, 0.002, respectively). In multiple linear regression analysis with APPL1 as the dependent variable, HOMA-IR (β=0.329, P=0.002) was included in the equation.
Patients with newly diagnosed T2DM have elevated serum APPL1 levels, suggesting the involvement of APPL1 in the development of T2DM.
探讨新诊断2型糖尿病（T2DM）患者血清APPL1水平，并分析其与体重指数（BMI）、腰臀比（WHR）、血压、空腹血糖、空腹血胰岛素、糖化血红蛋白（HbA1c）及胰岛素抵抗（HOMA-IR）的相关性。
采用酶联免疫吸附测定法（ELISA）检测22例正常对照者及63例新诊断T2DM患者的血清APPL1水平，并使用HOMA模型计算受试者的HOMA-IR。
与对照组相比，糖尿病患者血清APPL1水平显著升高（85.71±27.39对64.52±16.28 pg/ml，P<0.01），BMI、WHR、收缩压（SBP）、空腹血胰岛素（FINS）、LogHOMA-IR（P<0.01）及低密度脂蛋白胆固醇（LDL-C，P<0.05）也显著升高，而高密度脂蛋白胆固醇（HDL-C，P<0.01）降低。空腹血清APPL1水平与空腹血糖（FPG）、FINS及HOMA-IR呈正相关（r分别为0.215、0.297、0.334，P分别为0.014、0.006、0.002）。以APPL1为因变量的多元线性回归分析中，HOMA-IR（β=0.329，P=0.002）纳入方程。
新诊断T2DM患者血清APPL1水平升高，提示APPL1参与T2DM的发生发展。
