Caroprese Mariangela, Albenzio Marzia, Ciliberti Maria Giovanna, Francavilla Matteo, Sevi Agostino
Department of Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli, 25, 71122 Foggia, Italy.
Vet Immunol Immunopathol. 2012 Nov 15;150(1-2):27-35. doi: 10.1016/j.vetimm.2012.08.002. Epub 2012 Aug 13.
The aim of this study was to investigate the immunomodulatory and anti-inflammatory role of a mixture of phytosterols extracted from the microalga Dunaliella tertiolecta on peripheral blood mononuclear cells (PBMC) isolated from sheep. PBMC were treated to determine cell proliferation and cytokine production with different sterols: ergosterol (E), a mixture of eleven Algae sterols extracted and purified from D. tertiolecta (Algae Extract, AE), a mixture of ergosterol and 7-dehydroporiferasterol extracted and purified from D. tertiolecta (Purified Extract, PE). Cytokine production (TNF-α, IL-6, IL-1β, and IL-10) was evaluated after cell treatment with Concanavalin A (Con A) and lipopolysaccharide (LPS). The mixture of ergosterol and 7-dehydroporiferasterol extracted and purified from D. tertiolecta showed a suppressive effect on cell proliferation, and a reduction of pro-inflammatory cytokines production. Furthermore, a stimulatory effect on the production of the regulatory cytokine IL-10 was found. The immunosuppressive effect exerted by the mixture of ergosterol and 7-dehydroporiferasterol from D. tertiolecta was dose-dependent both in suppressing cell proliferation and in stimulating IL-10 production. Present results showed that the immunomodulatory and anti-inflammatory activities were more apparent in the purified extract characterized by the mixture of ergosterol and 7-dehydroporiferasterol, and might depend on the existence of a synergic effect of the structures of the two phytosterols. Furthermore, findings from our study suggest that the purified extract characterized by the mixture of ergosterol and 7-dehydroporiferasterol from D. tertiolecta could be used to reduce immune reactions resulting from inflammatory diseases in sheep production systems, and could have innovative implications on the modulation of sheep immune system when used as feed supplements.
本研究的目的是调查从微藻杜氏盐藻中提取的植物甾醇混合物对从绵羊分离的外周血单个核细胞（PBMC）的免疫调节和抗炎作用。用不同的甾醇处理PBMC以确定细胞增殖和细胞因子产生：麦角甾醇（E）、从杜氏盐藻中提取和纯化的11种藻类甾醇的混合物（藻类提取物，AE）、从杜氏盐藻中提取和纯化的麦角甾醇和7-脱氢海绵甾醇的混合物（纯化提取物，PE）。在用伴刀豆球蛋白A（Con A）和脂多糖（LPS）处理细胞后评估细胞因子产生（TNF-α、IL-6、IL-1β和IL-10）。从杜氏盐藻中提取和纯化的麦角甾醇和7-脱氢海绵甾醇的混合物对细胞增殖有抑制作用，并减少促炎细胞因子的产生。此外，还发现对调节性细胞因子IL-10的产生有刺激作用。从杜氏盐藻中提取的麦角甾醇和7-脱氢海绵甾醇混合物所发挥的免疫抑制作用在抑制细胞增殖和刺激IL-10产生方面均呈剂量依赖性。目前的结果表明，以麦角甾醇和7-脱氢海绵甾醇混合物为特征的纯化提取物中的免疫调节和抗炎活性更为明显，并且可能取决于两种植物甾醇结构的协同效应的存在。此外，我们研究的结果表明，以杜氏盐藻中麦角甾醇和7-脱氢海绵甾醇混合物为特征的纯化提取物可用于减少绵羊生产系统中炎症性疾病引起的免疫反应，并且用作饲料补充剂时可能对绵羊免疫系统的调节具有创新意义。
