Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR 72401, USA.
Bioresour Technol. 2012 Nov;124:485-8. doi: 10.1016/j.biortech.2012.08.050. Epub 2012 Aug 19.
Duckweed (Lemna minor) was grown in swine lagoon wastewater and Schenk & Hildebrandt medium with a growth rate of 3.5 and 14.1 g m(-2)day(-1) (dry basis), respectively detected. The rapid accumulation of starch in duckweed biomass (10-36%, w/w) was triggered by nutrient starvation or growing in dark with addition of glucose. The harvested duckweed biomass (from culture in wastewater) contained 20.3% (w/w) total glucan, 32.3% (w/w) proteins, trace hemicellulose and undetectable lignin. Without prior thermal-chemical pretreatment, up to 96.2% (w/w) of glucose could be enzymatically released from both the cellulose and starch fractions of duckweed biomass. The enzymatic hydrolysates could be efficiently fermented by two yeast strains (self-flocculating yeast SPSC01 and conventional yeast ATCC 24859) with a high ethanol yield of 0.485 g g(-1) (glucose).
浮萍（Lemna minor）在猪粪污水和 Schenk & Hildebrandt 培养基中生长，其生长速率分别为 3.5 和 14.1 g m(-2)day(-1)（干重）。浮萍生物量中淀粉的快速积累（10-36%，w/w）是由营养饥饿或在黑暗中添加葡萄糖生长引起的。收获的浮萍生物质（来自污水培养）含有 20.3%（w/w）总葡聚糖、32.3%（w/w）蛋白质、痕量半纤维素和不可检测的木质素。未经预先的热化学预处理，从浮萍生物质的纤维素和淀粉部分中可以酶解释放高达 96.2%（w/w）的葡萄糖。酶解产物可以由两种酵母菌株（自絮凝酵母 SPSC01 和传统酵母 ATCC 24859）高效发酵，葡萄糖的乙醇得率高达 0.485 g g(-1)。
