Department of Plant Ecophysiology, University of Lodz, Banacha Str. 12/16, 92-237, Lodz, Poland.
Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213, 90-924, Lodz, Poland.
Sci Rep. 2023 Oct 2;13(1):16533. doi: 10.1038/s41598-023-43576-y.
Considering the main objectives of a circular economy, Lemnaceae plants have great potential for different types of techniques to valorize their biomass for use in biofuel production. For this reason, scientific interest in this group of plants has increased in recent years. The aim of this study was to evaluate the effects of salt stress on the growth and development of S. polyrrhiza and the valorization of biomass for biofuel and energy production in a circular economy. Plants were grown in a variety of culture media, including standard 'Z' medium, tap water, 1% digestate from a biogas plant in Piaszczyna (54° 01' 21″ N, 17° 10' 19″ E), Poland) and supplemented with different concentrations of NaCl (from 25 to 100 mM). Plants were cultured under phytotron conditions at 24 °C. After 10 days of culture, plant growth, fresh and dry biomass, as well as physio-chemical parameters such as chlorophyll content index, gas exchange parameters (net photosynthesis, transpiration, stomatal conductance and intercellular CO concentration), chlorophyll fluorescence measurements were analyzed. After 10 days of the experiment, the percentage starch content of Spirodela shoot segments was determined. S. polyrrhiza was shown to have a high starch storage capacity under certain unfavorable growth conditions, such as salt stress and nutrient deficiency. In the W2 (50 mM NaCl) series, compared to the control (Control2), starch levels were 76% higher in shoots and 30% lower in roots. The analysis of the individual growth and development parameters of S. polyrrhiza plants in the experiment carried out indicates new possibilities for the use of this group of plants in biofuel and bioethanol production.
考虑到循环经济的主要目标，水鳖科植物具有很大的潜力，可以通过不同类型的技术来利用其生物质，用于生物燃料生产。出于这个原因，近年来科学界对这组植物的兴趣有所增加。本研究的目的是评估盐胁迫对紫萍生长和发育的影响，以及在循环经济中利用生物质生产生物燃料和能源。植物在多种培养基中生长，包括标准的 Z 培养基、自来水、来自波兰皮亚谢奇纳沼气厂的 1%消化物（54°01'21″N，17°10'19″E）和补充不同浓度的 NaCl（从 25 到 100 mM）。植物在 24°C 的人工气候室内培养。培养 10 天后，分析植物生长、鲜重和干重、生理生化参数（叶绿素含量指数、气体交换参数（净光合速率、蒸腾速率、气孔导度和胞间 CO 浓度）、叶绿素荧光测量。实验进行 10 天后，测定紫萍茎段的淀粉含量百分比。在某些不利的生长条件下，如盐胁迫和营养缺乏，紫萍表现出较高的淀粉储存能力。在 W2（50 mM NaCl）系列中，与对照（Control2）相比，茎中的淀粉含量增加了 76%，根中的淀粉含量降低了 30%。对实验中紫萍植物个体生长和发育参数的分析表明，该组植物在生物燃料和生物乙醇生产中有新的应用可能性。
