Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
Bioresour Technol. 2012 Nov;124:111-8. doi: 10.1016/j.biortech.2012.08.067. Epub 2012 Aug 26.
Different alkaline pretreatment methods (NaOH, NaOH+10% urea and aqueous ammonia) were optimized for maximum delignification of Saccharum spontaneum at 30°C. Maximum delignification were obtained as 47.8%, 51% and 48% from NaOH (7% NaOH, 48h, and 10% biomass loading), NaOH+urea (7% NaOH+10% urea, 48 h and 10% biomass loading) and 30% ammonia (40 days and 10% biomass loading) respectively. H(2)SO(4) 60% (v/v), 10% biomass loading at 30°C for 4h, were optimized conditions to solubilize the cellulose and hemicellulose from solid residue obtained after different optimized alkaline pretreatments. Slurry thus obtained was diluted to obtain final acid concentration of 10% (v/v) for real hydrolysis of cellulose and hemicellulose at 100°C for 1h. Among all pretreatment methods applied, the best result 0.58 g (85%) reducing sugars/g of initial biomass after acid hydrolysis was obtained from aqueous ammonia pretreated biomass. Scheffersomyces stipitis CBS6054 was used to ferment the hydrolysate; ethanol yield (Y(p/s)) and productivity (r(p)) were found to be 0.35 g/g and 0.22 g/L/h respectively.
不同的碱性预处理方法（NaOH、NaOH+10%尿素和氨水）在 30°C 下被优化，以实现甜高粱的最大脱木质素。从 NaOH（7%NaOH，48 小时，生物质负载量为 10%）、NaOH+尿素（7%NaOH+10%尿素，48 小时，生物质负载量为 10%）和 30%氨水中分别获得最大脱木质素率 47.8%、51%和 48%。H₂SO₄ 60%（v/v），10%生物质负载量，在 30°C 下反应 4 小时，是从不同优化碱性预处理后获得的固体残渣中溶解纤维素和半纤维素的最佳条件。由此得到的浆液被稀释，以获得最终酸浓度为 10%（v/v），用于在 100°C 下进行 1 小时的纤维素和半纤维素的实际水解。在所应用的所有预处理方法中，用氨水预处理的生物质在酸水解后得到的最佳还原糖产量为 0.58g（85%）/g 初始生物质。将酿酒酵母 CBS6054 用于发酵水解液；乙醇产率（Y（p/s））和生产率（r（p））分别为 0.35g/g 和 0.22g/L/h。
