Department of Obstetrics and Gynecology, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
Int J Gynaecol Obstet. 2012 Dec;119(3):234-8. doi: 10.1016/j.ijgo.2012.07.010. Epub 2012 Sep 15.
To determine the concentration of syncytiotrophoblast-derived microparticles (STBMs) in the maternal circulation and the rate of syncytiotrophoblast apoptosis in the placenta of patients with early-onset and late-onset severe pre-eclampsia.
A prospective case-control study was conducted that enrolled 15 women with early-onset severe pre-eclampsia, 15 women with late-onset severe pre-eclampsia, and 10 women with normal pregnancies. Plasma STBM levels were measured by enzyme-linked immunosorbent assay, while placental levels of active caspase-3 were determined by western blotting. Human umbilical vein endothelial cells (HUVECs) were cultured with STBMs and the proliferation and apoptosis rates of the HUVECs assessed.
Levels of STBMs in the early-onset group (71.2 ± 20.7 ng/mL) were significantly higher than those detected in the late-onset group (41.9 ± 29.7 ng/mL) and the control group (26.3 ± 11.2 ng/mL) (P<0.05). The amount of active caspase-3 was increased in the early-onset (0.85 ± 0.61) and late-onset groups (0.77 ± 0.46) relative to the control group (0.32 ± 0.15) (P<0.05). Proliferation of HUVECs was inhibited, while apoptosis was elevated, following co-culture with STBMs.
Shedding of STBMs into the maternal circulation occurs in greater amounts in early-onset pre-eclampsia than in late-onset pre-eclampsia.
测定早发型和晚发型重度子痫前期患者母体外周血中合体滋养细胞衍生的微粒（STBM）浓度和胎盘合体滋养细胞凋亡率。
前瞻性病例对照研究纳入了 15 例早发型重度子痫前期患者、15 例晚发型重度子痫前期患者和 10 例正常妊娠孕妇。采用酶联免疫吸附试验检测血浆 STBM 水平，Western blot 法检测胎盘组织中活性半胱氨酸天冬氨酸蛋白酶-3（caspase-3）的表达。将 STBM 与脐静脉内皮细胞（HUVEC）共培养，检测 HUVEC 的增殖和凋亡情况。
早发型组（71.2±20.7ng/ml）的 STBM 水平明显高于晚发型组（41.9±29.7ng/ml）和对照组（26.3±11.2ng/ml）（P<0.05）。早发型组（0.85±0.61）和晚发型组（0.77±0.46）的活性 caspase-3 表达量均高于对照组（0.32±0.15）（P<0.05）。与 STBM 共培养后，HUVEC 的增殖受到抑制，凋亡增加。
早发型子痫前期患者母体外周血中 STBM 的释放量多于晚发型子痫前期患者。
