Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Anal Chim Acta. 2012 Oct 17;747:1-6. doi: 10.1016/j.aca.2012.08.008. Epub 2012 Aug 14.
Cations affect the topology and enzymatic proficiency of most macromolecular catalysts but the role of cations in DNAzyme peroxidation reactions remains unresolved. Herein, we use statistical methods (ANOVA, t-test and Wilcoxon Mann-Whitney non-parametric test) to demonstrate that there are strong associations between cations, DNAzyme topology, peroxidation substrate and peroxidation rates of G-quadruplex peroxidises. Ammonium cation was found to be superior to all tested cations, including potassium. A t-test indicated that NH(4)(+) was better than K(+) with a p-value=0.05. Interestingly, the nature of the peroxidation substrate employed affected the dependence of peroxidation rate on the cation present and of the three substrates tested, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), tyramine and 3,3',5,5'-tetramethylbenzidine (TMB), ABTS was the most sensitive to the nature of cation present.
阳离子会影响大多数大分子催化剂的拓扑结构和酶活性，但阳离子在 DNA 酶过氧化物反应中的作用仍未得到解决。在此，我们使用统计方法（方差分析、t 检验和 Wilcoxon 曼-惠特尼非参数检验）来证明阳离子、DNA 酶拓扑结构、过氧化物底物和 G-四链体过氧化物的过氧化物率之间存在很强的关联。铵阳离子被发现优于所有测试的阳离子，包括钾。t 检验表明，NH4+比 K+更好，p 值=0.05。有趣的是，所使用的过氧化物底物的性质会影响过氧化物率对存在的阳离子的依赖性，在所测试的三种底物中，2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）、酪胺和 3,3',5,5'-四甲基联苯胺（TMB），ABTS 对阳离子的性质最为敏感。
