Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.
Anal Chim Acta. 2012 Oct 17;747:7-18. doi: 10.1016/j.aca.2012.08.004. Epub 2012 Aug 21.
Protein phosphorylation is one of the most important post-translational modifications. Phosphorylated peptides are present in low abundance in blood serum but play a vital role in regulatory mechanisms and may serve as casual factors in diseases. The enrichment and analysis of phosphorylated peptides directly from human serum and mapping the phosphorylation sites is a challenging task. Versatile nanocomposites of different materials have been synthesized using simple but efficient methodologies for their enrichment. The nanocomposites include magnetic, coated, embedded as well as chemically derivatized materials. Different base materials such as polymers, carbon based and metal oxides are used. The comparison of nanocomposites with respective nanoparticles provides sufficient facts about their efficiency in terms of loading capacity and capture efficiency. The cost for preparing them is low and they hold great promise to be used as chromatographic materials for phosphopeptide enrichment. This review gives an overview of different nanocomposites in phosphoproteomics, discussing the improved efficiency than the individual counterparts and highlighting their significance in phosphopeptide enrichment.
蛋白质磷酸化是最重要的翻译后修饰之一。磷酸化肽在血清中的含量较低，但在调节机制中起着至关重要的作用，并且可能作为疾病的偶然因素。直接从人血清中富集和分析磷酸化肽并绘制磷酸化位点是一项具有挑战性的任务。已经使用简单但有效的方法合成了不同材料的多功能纳米复合材料，用于其富集。纳米复合材料包括磁性、涂层、嵌入式和化学衍生材料。使用不同的基础材料，如聚合物、碳基和金属氧化物。纳米复合材料与相应的纳米颗粒的比较提供了关于其在载量和捕获效率方面的效率的充分事实。制备它们的成本低，它们有望作为用于磷酸肽富集的色谱材料。本综述概述了磷酸蛋白质组学中的不同纳米复合材料，讨论了其比单个对应物提高的效率，并强调了它们在磷酸肽富集中的重要性。
