RLP068 的抗菌光动力疗法可杀灭耐甲氧西林金黄色葡萄球菌，并改善感染皮肤擦伤小鼠模型的伤口愈合
Antimicrobial photodynamic therapy with RLP068 kills methicillin-resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion.
机构信息
Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USA.
出版信息
J Biophotonics. 2013 Sep;6(9):733-42. doi: 10.1002/jbio.201200121. Epub 2012 Sep 14.
Abstract
Photodynamic therapy (PDT) is an alternative treatment for infections that can kill drug resistant bacteria without damaging host-tissue. In this study we used bioluminescent methicillin-resistant Staphylococcus aureus, in a mouse skin abrasion model, to investigate the effect of PDT on bacterial inactivation and wound healing. RLP068/Cl, a tetracationic Zn(II)phthalocyanine derivative and toluidine blue (TBO) were used. The light-dose response of PDT to kill bacteria in vivo and the possible recurrence in the days post-treatment were monitored by real-time bioluminescence imaging, and wound healing by digital photography. The results showed PDT with RLP068/Cl (but not TBO) was able to kill bacteria, to inhibit bacterial re-growth after the treatment and to significantly accelerate the wound healing process (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
摘要
光动力疗法（PDT）是一种替代治疗感染的方法，它可以杀死耐药细菌而不损伤宿主组织。在这项研究中，我们使用生物发光性耐甲氧西林金黄色葡萄球菌（methicillin-resistant Staphylococcus aureus），在小鼠皮肤擦伤模型中，研究 PDT 对细菌失活和伤口愈合的影响。我们使用了四阳离子锌（Ⅱ）酞菁衍生物 RLP068/Cl 和甲苯胺蓝（toluidine blue，TBO）。通过实时生物发光成像监测 PDT 对体内细菌的杀伤作用及其在治疗后数天内的潜在复发情况，并用数码摄影监测伤口愈合情况。结果表明，RLP068/Cl 的 PDT（而非 TBO）能够杀死细菌，抑制治疗后的细菌再生长，并显著加速伤口愈合过程（© 2013 WILEY-VCH Verlag GmbH & Co. KGaA，Weinheim）。
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