用于植原体的DAPI染色和荧光显微镜技术。

DAPI staining and fluorescence microscopy techniques for phytoplasmas.

作者信息

Andrade Nancy M, Arismendi Nolberto L

机构信息

Institute of Production and Plant Protection, Universidad Austral de Chile, Valdivia, Chile.

出版信息

Methods Mol Biol. 2013;938:115-21. doi: 10.1007/978-1-62703-089-2_10.

DOI:10.1007/978-1-62703-089-2_10

PMID:22987410

Abstract

The 4',6-diamidino-2-phenylindole (DAPI) stain technique is a simple method that was developed for confirming the presence of phytoplasmas in hand-cut or freezing microtome sections of infected tissues. DAPI binds AT-rich DNA preferentially, so that phytoplasmas, localized among phloem cells, can be visualized in a fluorescence microscope. The procedure is quick, easy to use, inexpensive, and can be used as a preliminary or quantitative method to detect or quantify phytoplasma-like bodies in infected plants.

摘要

4',6-二脒基-2-苯基吲哚（DAPI）染色技术是一种简单的方法，它被开发用于在感染组织的手工切片或冰冻切片中确认植原体的存在。DAPI优先结合富含AT的DNA，这样，位于韧皮部细胞间的植原体就能在荧光显微镜下被观察到。该程序快速、易于使用且成本低廉，可作为检测或定量感染植物中类植原体的初步方法或定量方法。