用肝素包被的核膜的荧光染色。
Fluorescent staining of nuclear envelope coated with heparin.
作者信息
Grossgebauer K
出版信息
Microsc Acta. 1980 Mar;83(1):49-54.
PMID:6156382
Abstract
Naturally and artificially devitalized cells of various origin can be loaded with heparin and subsequently stained with DAPI, a newer fluorochrome (4',6-diamidino-2-phenylindole), leading to a bright yellow fluorescence of the nuclear envelope. Depending on the cell type it is also possible to coat the outer cell membrane with heparin or other sulphated mucopolysaccharides and to achieve similar staining phenomena with DAPI.
摘要
各种来源的天然和人工失活细胞可装载肝素，随后用一种较新的荧光染料4',6-二脒基-2-苯基吲哚（DAPI）染色，从而使核膜发出亮黄色荧光。根据细胞类型，也可以用肝素或其他硫酸化粘多糖包被细胞外膜，并通过DAPI实现类似的染色现象。
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