植原体的Tuf和secY基因PCR扩增及基因分型

Tuf and secY PCR amplification and genotyping of phytoplasmas.

作者信息

Foissac Xavier, Danet Jean-Luc, Malembic-Maher Sylvie, Salar Pascal, Safářová Dana, Válová Pavla, Navrátil Milan

机构信息

UMR1332 Fruit Biology and Pathology, INRA, University of Bordeaux, Villenave d'Ornon, France.

出版信息

Methods Mol Biol. 2013;938:189-204. doi: 10.1007/978-1-62703-089-2_16.

DOI:10.1007/978-1-62703-089-2_16

PMID:22987416

Abstract

Tuf and secY genotyping techniques have been developed to distinguish phytoplasma strains. Tuf polymerase chain reaction sequence analyses are available for phytoplasma taxonomic groups 16SrI, 16SrV, 16SrXII-A, and XII-B. In addition to their use to confirm the taxonomic status of phytoplasma strains, they allow the spread of phytoplasma strains in host plants and insect vectors to be traced. SecY is more variable than tuf and is therefore more discriminatory than tuf, but secY and tuf phylogenies show congruence.

摘要

已开发出Tuf和secY基因分型技术来区分植原体菌株。Tuf聚合酶链反应序列分析可用于植原体分类群16SrI、16SrV、16SrXII - A和XII - B。除了用于确认植原体菌株的分类地位外，它们还能追踪植原体菌株在寄主植物和昆虫介体中的传播。SecY比tuf更具变异性，因此比tuf更具鉴别力，但secY和tuf系统发育显示出一致性。