用于植原体鉴定的DNA条形码技术。

DNA bar-coding for phytoplasma identification.

作者信息

Makarova Olga, Contaldo Nicoletta, Paltrinieri Samanta, Bertaccini Assunta, Nyskjold Henriette, Nicolaisen Mogens

机构信息

Department of Agroecology, Aarhus University, Slagelse, Denmark.

出版信息

Methods Mol Biol. 2013;938:301-17. doi: 10.1007/978-1-62703-089-2_26.

DOI:10.1007/978-1-62703-089-2_26

PMID:22987426

Abstract

Phytoplasma identification has proved difficult due to their inability to be maintained in vitro. DNA barcoding is an identification method based on comparison of a short DNA sequence with known sequences from a database. A DNA barcoding tool has been developed for phytoplasma identification. While other sequence-based methods may be well adapted to identification of particular strains of phytoplasmas, often they cannot be used for the simultaneous identification of phytoplasmas from different groups. The phytoplasma DNA barcoding protocol in this chapter, based on the tuf and 16SrRNA genes, can be used to identify the following phytoplasma groups: 16SrI, 16SrII, 16SrIII, 16SrIV, 16SrV, 16SrVI, 16SrVII, 16SrIX, 16SrX, 16SrXI, 16SrXII, 16SrXV, 16SrXX, 16SrXXI.

摘要

由于植原体无法在体外培养，其鉴定工作颇具难度。DNA条形码技术是一种基于将短DNA序列与数据库中已知序列进行比对的鉴定方法。现已开发出一种用于植原体鉴定的DNA条形码工具。虽然其他基于序列的方法可能非常适合鉴定特定的植原体菌株，但它们通常无法用于同时鉴定来自不同组的植原体。本章基于tuf和16SrRNA基因的植原体DNA条形码协议可用于鉴定以下植原体组：16SrI、16SrII、16SrIII、16SrIV、16SrV、16SrVI、16SrVII、16SrIX、16SrX、16SrXI、16SrXII、16SrXV、16SrXX、16SrXXI。