用于普遍检测和鉴定植原体的微阵列。

Microarrays for universal detection and identification of phytoplasmas.

作者信息

Nicolaisen Mogens, Nyskjold Henriette, Bertaccini Assunta

机构信息

Department of Agroecology, Aarhus University, Slagelse, Denmark.

出版信息

Methods Mol Biol. 2013;938:223-32. doi: 10.1007/978-1-62703-089-2_19.

DOI:10.1007/978-1-62703-089-2_19

PMID:22987419

Abstract

Detection and identification of phytoplasmas is a laborious process often involving nested PCR followed by restriction enzyme analysis and fine-resolution gel electrophoresis. To improve throughput, other methods are needed. Microarray technology offers a generic assay that can potentially detect and differentiate all types of phytoplasmas in one assay. The present protocol describes a microarray-based method for identification of phytoplasmas to 16Sr group level.

摘要

植原体的检测与鉴定是一个费力的过程，通常包括巢式PCR，随后进行限制性酶切分析和高分辨率凝胶电泳。为了提高通量，需要其他方法。微阵列技术提供了一种通用检测方法，有可能在一次检测中检测和区分所有类型的植原体。本方案描述了一种基于微阵列的方法，用于将植原体鉴定到16Sr组水平。