Department of Otorhinolaryngology, Saarland University Medical Center, Homburg/Saar, Germany.
Head Neck. 2013 Oct;35(10):1431-8. doi: 10.1002/hed.23147. Epub 2012 Sep 18.
Despite numerous studies, the tumor biology of pleomorphic adenomas, the most common salivary gland tumors, is still not completely defined. In order to identify further candidate genes important for tumor biology of pleomorphic adenomas, extended cytogenetic and molecular analysis are mandatory.
We performed a detailed molecular cytogenetic analysis using comparative genomic hybridization (CGH) followed by fluorescence in situ hybridization (FISH) with probes for chromosome X, 16p, 17, and 20 on a large cohort of pleomorphic adenomas (n = 29).
We could confirm previously described deletions in pleomorphic adenomas affecting 16p, 17, 20q, and 22 by FISH and/or CGH analysis. Moreover, our CGH study revealed novel candidate regions on 8p23.1pter, 9p, 10q25.1q25.3, and 11q24qter in the series of analyzed pleomorphic adenomas.
Our present study reveals new insights in novel candidate regions implicated in pleomorphic adenoma tumorigenesis which should be considered in further molecular studies.
尽管已有大量研究，但多形性腺瘤（最常见的唾液腺肿瘤）的肿瘤生物学仍未完全阐明。为了进一步确定多形性腺瘤肿瘤生物学中重要的候选基因，有必要进行扩展的细胞遗传学和分子分析。
我们使用比较基因组杂交（CGH）进行了详细的分子细胞遗传学分析，然后使用针对染色体 X、16p、17 和 20 的荧光原位杂交（FISH）探针对一大群多形性腺瘤（n = 29）进行了分析。
我们通过 FISH 和/或 CGH 分析证实了先前描述的多形性腺瘤中影响 16p、17、20q 和 22 的缺失。此外，我们的 CGH 研究还揭示了所分析的多形性腺瘤系列中 8p23.1pter、9p、10q25.1q25.3 和 11q24qter 上的新候选区域。
本研究揭示了多形性腺瘤发生中涉及的新候选区域的新见解，这些区域应在进一步的分子研究中加以考虑。
