Division of Molecular and Experimental Surgery, Clinical Center Erlangen, Erlangen, Germany.
Int J Cancer. 2013 Apr 15;132(8):1954-8. doi: 10.1002/ijc.27849. Epub 2012 Oct 12.
Kaposi's sarcoma (KS) is an endothelial cell-derived tumor. Investigations of the molecular mechanisms of KS pathogenesis and the identification of drugs for treatment of KS depend critically on valid cell-culture models. Two major immortalized cell lines are available for KS research. Recently, the KS cell line KS Y-1 has been shown to be cross-contaminated with the T24 urinary bladder cancer cell line (ATCC HTB-4). Here, we show by short tandem repeat profiling that the second KS cell line, SLK, is indistinguishable from the clear-cell renal-cell carcinoma cell line Caki-1. Immunocytochemical detection of cytokeratin expression confirmed the epithelial-cell origin of SLK cells. Our findings indicate that SLK cells are not of endothelial origin and should not be used in future studies as a model for KS-derived endothelial tumor cells. We suggest that in the future, more attention needs to be paid to the authenticity of cells in lines derived from human tissues.
卡波氏肉瘤（KS）是一种源自内皮细胞的肿瘤。对 KS 发病机制的分子机制的研究以及用于治疗 KS 的药物的鉴定都严重依赖于有效的细胞培养模型。有两种主要的永生化细胞系可用于 KS 研究。最近，KS 细胞系 KS Y-1 已被证明与 T24 膀胱癌细胞系（ATCC HTB-4）交叉污染。在这里，我们通过短串联重复序列分析表明，第二种 KS 细胞系 SLK 与透明细胞肾细胞癌细胞系 Caki-1 无法区分。免疫细胞化学检测细胞角蛋白表达证实了 SLK 细胞的上皮细胞起源。我们的研究结果表明，SLK 细胞不是内皮细胞起源，不应在未来的研究中用作 KS 衍生的内皮肿瘤细胞的模型。我们建议，在未来，需要更加关注源自人体组织的细胞系中细胞的真实性。
