Lunardi-Iskandar Y, Bryant J L, Zeman R A, Lam V H, Samaniego F, Besnier J M, Hermans P, Thierry A R, Gill P, Gallo R C
Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Nature. 1995 May 4;375(6526):64-8. doi: 10.1038/375064a0.
Kaposi's sarcoma (KS) occurs more often in men than in women and HIV-1-associated KS has a high occurrence in homosexual men (over 30%). Most cultures of KS tumours yield cells with properties of hyperplastic (not malignant) endothelial cells under the control of several cytokines. The role of HIV-1 may be in promoting high levels of some cytokines and providing stimulation to angiogenesis by the HIV-1 Tat protein, which synergizes with basic fibroblast growth factor in promoting these effects. Here we describe an immortalized AIDS-KS cell line (KS Y-1) and show that these cells produce malignant metastatic tumours in nude mice and are killed in vitro and in vivo (apparently by apoptosis) by a pregnancy hormone, the beta-chain of human chorionic gonadotropin. Similarly, chorionic gonadotropin kills KS SLK, cells from another neoplastic cell line (established from a non-HIV-associated KS), as well as the hyperplastic KS cells from clinical specimens grown in short-term culture, but does not kill normal endothelial cells. These results provide evidence that KS can evolve into a malignancy and have implications for the hormonal treatment of this tumour.
卡波西肉瘤（KS）在男性中的发病率高于女性，且与HIV-1相关的KS在同性恋男性中发病率很高（超过30%）。大多数卡波西肉瘤肿瘤培养物在几种细胞因子的控制下产生具有增生性（非恶性）内皮细胞特性的细胞。HIV-1的作用可能在于促进某些细胞因子的高水平表达，并通过HIV-1反式激活蛋白刺激血管生成，该蛋白在促进这些作用方面与碱性成纤维细胞生长因子协同作用。在此，我们描述了一种永生化的艾滋病相关卡波西肉瘤细胞系（KS Y-1），并表明这些细胞在裸鼠中产生恶性转移性肿瘤，且在体外和体内（显然通过凋亡）被一种妊娠激素——人绒毛膜促性腺激素β链杀死。同样，绒毛膜促性腺激素可杀死KS SLK，即来自另一种肿瘤细胞系（由非HIV相关的卡波西肉瘤建立）的细胞，以及短期培养的临床标本中的增生性卡波西肉瘤细胞，但不会杀死正常内皮细胞。这些结果证明卡波西肉瘤可演变为恶性肿瘤，并对该肿瘤的激素治疗具有启示意义。
