培养的卡波西肉瘤细胞系表达因子ⅩⅢa、CD14和血管细胞黏附分子-1，但不表达因子Ⅷ或内皮细胞白细胞黏附分子-1。

Cultured Kaposi's sarcoma cell lines express factor XIIIa, CD14, and VCAM-1, but not factor VIII or ELAM-1.

作者信息

Huang Y Q, Friedman-Kien A E, Li J J, Nickoloff B J

机构信息

Department of Dermatology, New York, NY, University School of Medicine.

出版信息

Arch Dermatol. 1993 Oct;129(10):1291-6.

PMID:7692824

Abstract

BACKGROUND AND DESIGN

Historically, the Kaposi's sarcoma (KS) tumor cell was generally thought to be derived from endothelial cells. However, by immunohistochemical staining of lesions, many spindle-shaped cells in KS express factor XIIIa, suggesting that they may be related to dermal dendrocytes, a perivascular cell of monocyte/macrophage lineage. To extend our observations on tissue sections of lesions, four different established KS-derived cell lines before and after cytokine exposure, as well as biopsy specimens of KS lesions, were studied for the expression of endothelial cell and monocyte/macrophage antigens.

RESULTS

Multipassaged KS tumor cells grew predominantly as spindle-shaped cells. By immunohistochemical staining, all four KS-derived cell cultures were factor XIIIa and intercellular adhesion molecule 1 positive. Three KS-derived cell cultures were CD14 and vascular cell adhesion molecule 1 positive. Expression of factor XIIIa was upregulated by interferon gamma. By polymerase chain reaction (PCR) analysis, expression of CD4 mRNA was also detected in all four KS-derived cell cultures. Expression of these monocyte/macrophage markers (factor XIIIa, CD14, and vascular cell adhesion molecule 1) on KS in vitro was also detected on spindle cells of KS lesions in vivo. Endothelial leukocyte adhesion molecule 1 and factor VIII expression was not detected on KS-derived cells in vitro before or after exposure to interferon gamma or tumor necrosis factor alpha. While cultured KS cells strongly expressed smooth-muscle alpha-actin, no detection was observed on spindle-shaped cells in vivo. Conversely, while cultured KS cells were devoid of CD34, the tissue biopsy specimens contain strongly positive spindle-shaped cells. Our results demonstrate that while KS cell lines express one antigen shared by endothelial cells, ie, vascular cell adhesion molecule 1, they lack several other endothelial cell markers such as factor VIII and endothelial leukocyte adhesion molecule 1, either before or after cytokine activation.

CONCLUSIONS

Kaposi's sarcoma cell lines express several antigens linking them to dermal dendrocytes such as factor XIIIa, CD4, and CD14.

摘要

背景与设计

从历史上看，卡波西肉瘤（KS）肿瘤细胞通常被认为源自内皮细胞。然而，通过对病变进行免疫组织化学染色，KS中的许多梭形细胞表达因子ⅩⅢa，这表明它们可能与真皮树突状细胞有关，真皮树突状细胞是单核细胞/巨噬细胞谱系的一种血管周围细胞。为了扩展我们对病变组织切片的观察，我们研究了四种不同的已建立的KS衍生细胞系在细胞因子暴露前后以及KS病变活检标本中内皮细胞和单核细胞/巨噬细胞抗原的表达情况。

结果

多次传代的KS肿瘤细胞主要以梭形细胞形式生长。通过免疫组织化学染色，所有四种KS衍生细胞培养物均为因子ⅩⅢa和细胞间黏附分子1阳性。三种KS衍生细胞培养物为CD14和血管细胞黏附分子1阳性。干扰素γ可上调因子ⅩⅢa的表达。通过聚合酶链反应（PCR）分析，在所有四种KS衍生细胞培养物中也检测到了CD4 mRNA的表达。这些单核细胞/巨噬细胞标志物（因子ⅩⅢa、CD14和血管细胞黏附分子1）在体外KS中的表达也在体内KS病变的梭形细胞上检测到。在体外，无论是在暴露于干扰素γ或肿瘤坏死因子α之前还是之后，KS衍生细胞上均未检测到内皮白细胞黏附分子1和因子Ⅷ的表达。虽然培养的KS细胞强烈表达平滑肌α-肌动蛋白，但在体内的梭形细胞上未观察到表达。相反，虽然培养的KS细胞缺乏CD34，但组织活检标本中含有强阳性的梭形细胞。我们的结果表明，虽然KS细胞系表达一种内皮细胞共有的抗原，即血管细胞黏附分子1，但它们在细胞因子激活之前或之后缺乏其他几种内皮细胞标志物，如因子Ⅷ和内皮白细胞黏附分子1。