Hospices Civils de Lyon, Hôpital Edouard Herriot, Laboratoire d'Immunologie, Lyon F-69003, France.
Cytometry B Clin Cytom. 2013 Jan-Feb;84(1):59-62. doi: 10.1002/cyto.b.21043. Epub 2012 Sep 14.
Diminished expression of human leukocyte antigen DR on circulating monocytes (mHLA-DR) is a reliable indicator of immunosuppression in critically ill patients, predictive of both adverse outcome and septic complications. The objective of the present work was to test, in an inter-laboratory clinical study, a standardized protocol for mHLA-DR measurement by flow cytometry.
mHLA-DR was assessed in fresh whole blood according to a standardized staining protocol. Cells were analyzed on different flow cytometers (FC500, Navios, FACS Canto II) in different laboratories (Lyon and Grenoble). Results were expressed as numbers of antibodies bound per cell (AB/C).
Correlations between results were excellent (Pearson and interclass correlation coefficients > 0.98). Coefficients of variations for intra-assay precision ranged from 1.9 to 3.2%.
The present report highlights the robustness of this standardized flow cytometric protocol for mHLA-DR measurement in multicentric clinical studies.
循环单核细胞中人白细胞抗原 DR 表达减少（mHLA-DR）是危重病患者免疫抑制的可靠指标，可预测不良结局和脓毒症并发症。本研究旨在通过流式细胞术检测 mHLA-DR 的标准化方案，在实验室间临床研究中进行测试。
根据标准化染色方案评估新鲜全血中的 mHLA-DR。在不同实验室（里昂和格勒诺布尔）的不同流式细胞仪（FC500、Navios、FACS Canto II）上分析细胞。结果以每个细胞结合的抗体数量（AB/C）表示。
结果之间的相关性非常好（Pearson 和组内相关系数>0.98）。日内精密度的变异系数范围为 1.9%至 3.2%。
本报告强调了这种用于 mHLA-DR 测量的标准化流式细胞术方案在多中心临床研究中的稳健性。
