Departamento de Engenharia Têxtil, Universidade do Minho, Campus de Azurém, 4800-058 Guimarães, Portugal.
J Biomed Mater Res B Appl Biomater. 2012 Nov;100(8):2269-78. doi: 10.1002/jbm.b.32797. Epub 2012 Sep 15.
The Ca(2+)-induced cold gelation technique was found suitable to prepare highly porous biodegradable scaffolds based on bovine serum albumin (BSA) and alpha-casein from bovine milk for tissue engineering. A 2(3) full factorial design was used to study the influence and impact of each factor on the several responses of the scaffolds. In vitro degradation (ID), swelling ratio (SR), porosity (PO), and pore size (PS) as well cytotoxicity (CT) were evaluated and shown to be dependent on the pH of sample preparation and on the amount of BSA and casein present, making these scaffolds tunable structures. Under optimized working conditions (4.19% of BSA, 0.69% of Casein, pH 7.07), the ID attained was 37.97%, the SR observed was 11.87, the PO was 82.11%, the PS measured was 180.63 μm at surface, and 175.91 μm at fracture, whereas maximum cell viability was 84% in comparison to controls. Moreover, the scaffold supported cell adhesion and proliferation. These results, consistent with the prediction by the experimental design approach, support the use of this methodology to develop tunable scaffolds for tissue engineering using the Ca(2+)-induced cold gelation.
采用 Ca(2+)-诱导的低温凝胶技术，以牛血清白蛋白（BSA）和牛乳中的α-酪蛋白为原料，制备了用于组织工程的高度多孔可生物降解支架。采用 2(3)完全析因设计研究了每个因素对支架的几个响应的影响和作用。体外降解（ID）、溶胀比（SR）、孔隙率（PO）和孔径（PS）以及细胞毒性（CT）的评估结果表明，这些支架的结构具有可调性，取决于样品制备的 pH 值以及存在的 BSA 和酪蛋白的量。在优化的工作条件（4.19%BSA、0.69%Casein、pH7.07）下，ID 达到 37.97%，SR 为 11.87，PO 为 82.11%，表面 PS 为 180.63μm，断裂 PS 为 175.91μm，而与对照组相比，细胞活力最高可达 84%。此外，支架支持细胞黏附和增殖。这些结果与实验设计方法的预测一致，支持使用该方法开发用于组织工程的 Ca(2+)-诱导低温凝胶的可调支架。
