Nagahiro S, Takada A, Diksic M, Sourkes T L, Missala K, Yamamoto Y L
Department of Neurology and Neurosurgery, Montreal Neurological Institute and Hospital, Quebec, Canada.
J Cereb Blood Flow Metab. 1990 Jan;10(1):13-21. doi: 10.1038/jcbfm.1990.2.
We describe here a practical autoradiographic method to estimate the rate of serotonin synthesis in brain. A two-time point method (60 and 150 min after injection of alpha-[14C]methyl-L-tryptophan) was first evaluated in 14 normal rats (7 at each time point). After this the method was tested in lithium-treated rats. In normal rats the rate of serotonin synthesis measured by the two-time point method generally correlated with known concentrations of tryptophan hydroxylase. The rate of synthesis in lithium-treated rats was compared with that in sham-treated rats (NaCl treatment). The results showed a significant increase in the synthesis rate in some cerebral structures. The greatest increases in the serotonin synthesis rate, attributable to the lithium treatment, were observed in the parietal cortex (52%) and caudate nucleus (47%). This is the first investigation to demonstrate, with autoradiographic resolution (approximately 100 microns), the differential changes in the rate of serotonin synthesis in the brain. Lithium had no significant effect on the rate of synthesis in the pineal gland.
我们在此描述一种用于估计脑内5-羟色胺合成速率的实用放射自显影方法。首先在14只正常大鼠（每个时间点7只）中评估双时间点法（注射α-[¹⁴C]甲基-L-色氨酸后60分钟和150分钟）。在此之后，该方法在锂处理的大鼠中进行了测试。在正常大鼠中，通过双时间点法测得的5-羟色胺合成速率通常与已知的色氨酸羟化酶浓度相关。将锂处理大鼠的合成速率与假处理大鼠（氯化钠处理）的合成速率进行比较。结果显示，在一些脑结构中合成速率有显著增加。锂处理导致的5-羟色胺合成速率增加幅度最大的是顶叶皮质（52%）和尾状核（47%）。这是首次通过放射自显影分辨率（约100微米）证明脑内5-羟色胺合成速率的差异变化。锂对松果体的合成速率没有显著影响。
