Lu Rufei, Robertson Julie M, Bruner Benjamin F, Guthridge Joel M, Neas Barbara R, Nath Swapan K, Kelly Jennifer A, Moser Sivils Kathy L, Chakravarty Eliza F, Kamen Diane L, Gilkeson Gary S, Wallace Daniel J, Weisman Michael H, Scofield R Hal, Harley John B, James Judith A
Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA ; Department of Medicine and Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Autoimmune Dis. 2012;2012:819634. doi: 10.1155/2012/819634. Epub 2012 Sep 4.
Purpose. This study evaluates high-throughput autoantibody screening and determines associated systemic lupus erythematosus (SLE) clinical features in a large lupus cohort. Methods. Clinical and demographic information, along with serum samples, were obtained from each SLE study participant after appropriate informed consent. Serum samples were screened for 10 distinct SLE autoantibody specificities and examined for association with SLE ACR criteria and subcriteria using conditional logistic regression analysis. Results. In European-American SLE patients, autoantibodies against 52 kD Ro and RNP 68 are independently enriched in patients with lymphopenia, anti-La, and anti-ribosomal P are increased in patients with malar rash, and anti-dsDNA and anti-Sm are enriched in patients with proteinuria. In African-American SLE patients, cellular casts associate with autoantibodies against dsDNA, Sm, and Sm/nRNP. Conclusion. Using a high-throughput, bead-based method of autoantibody detection, anti-dsDNA is significantly enriched in patienets with SLE ACR renal criteria as has been previously described. However, lymphopenia is associated with several distinct autoantibody specificities. These findings offer meaningful information to allow clinicians and clinical investigators to understand which autoantibodies correlate with select SLE clinical manifestations across common racial groups using this novel methodology which is expanding in clinical use.
目的。本研究评估高通量自身抗体筛查，并确定一大群狼疮患者中相关的系统性红斑狼疮（SLE）临床特征。方法。在获得适当的知情同意后，从每位SLE研究参与者处获取临床和人口统计学信息以及血清样本。对血清样本进行10种不同的SLE自身抗体特异性筛查，并使用条件逻辑回归分析检查其与SLE美国风湿病学会（ACR）标准及次要标准的相关性。结果。在欧美SLE患者中，抗52 kD Ro和核糖核蛋白（RNP）68自身抗体在淋巴细胞减少的患者中独立富集，抗La和抗核糖体P在有颊部皮疹的患者中增加，抗双链DNA（dsDNA）和抗Sm在有蛋白尿的患者中富集。在非裔美国SLE患者中，细胞管型与抗dsDNA、抗Sm和抗Sm/nRNP自身抗体相关。结论。使用基于微珠的高通量自身抗体检测方法，抗dsDNA在符合SLE ACR肾脏标准的患者中显著富集，正如先前所述。然而，淋巴细胞减少与几种不同的自身抗体特异性相关。这些发现提供了有意义的信息，使临床医生和临床研究人员能够使用这种在临床应用中不断扩展的新方法，了解哪些自身抗体与不同种族群体中特定的SLE临床表现相关。
