Ohm T G, Müller H, Ulfig N, Braak E
Zentrum der Morphologie, J.W. Goethe-Universität, Frankfurt, Federal Republic of Germany.
J Comp Neurol. 1990 Jan 1;291(1):1-8. doi: 10.1002/cne.902910102.
This study examines the distribution and morphological characteristics of glutamic-acid-decarboxylase-like (GAD)- and parvalbumin-like (PA)-immunoreactive structures in the olfactory bulb of the human adult. GAD-immunoreactive somata occurred in the glomerular layer, the external granule cell layer, the more superficial portion of the external plexiform layer, and the internal granule cell layer. The cells were small- to medium-sized. Demonstration of lipofuscin pigment revealed the presence of unpigmented as well as pigmented neurons, thus suggesting the existence of two subpopulations of GAD-positive neurons. GAD-immunoreactive puncta and/or fibers were mainly seen in the periglomerular region and the internal granule cell layer. All other layers of the bulb, as well as the intrabulbar portion of the anterior olfactory nucleus, displayed considerably less of these puncta and/or fibers. The olfactory nerve layer remained practically clear of immunoreactive material. PA-immunoreactive somata occurred in the glomerular layer and both the external and internal granule cell layer. Only a small number of immunoreactive nerve cells were encountered within the white matter or the olfactory tract. Most PA-positive neurons displayed characteristics of short axon cells whereas a few others resembled van Gehuchten cells. All of the PA-immunoreactive neurons were devoid of lipofuscin pigment. Immunoreactive puncta and fibers were present in all layers though predominating in the periglomerular region, the olfactory nerve layer, and the internal granule cell layer. The intrabulbar portions of the anterior olfactory nucleus did not show any immunoreactive structures.
本研究考察了成年人类嗅球中谷氨酸脱羧酶样（GAD）和小白蛋白样（PA）免疫反应性结构的分布及形态特征。GAD免疫反应性胞体出现在肾小球层、外颗粒细胞层、外丛状层较浅部分以及内颗粒细胞层。细胞为中小型。脂褐素色素的显示揭示了未着色以及着色神经元的存在，因此提示存在两个GAD阳性神经元亚群。GAD免疫反应性小点和/或纤维主要见于肾小球周围区域和内颗粒细胞层。嗅球的所有其他层以及前嗅核的球内部分，这些小点和/或纤维的显示明显较少。嗅神经层实际上没有免疫反应性物质。PA免疫反应性胞体出现在肾小球层以及外颗粒细胞层和内颗粒细胞层。在白质或嗅束中仅遇到少量免疫反应性神经细胞。大多数PA阳性神经元表现出短轴突细胞的特征，而其他一些则类似于范·格胡滕细胞。所有PA免疫反应性神经元均无脂褐素色素。免疫反应性小点和纤维存在于所有层中，尽管在肾小球周围区域、嗅神经层和内颗粒细胞层中占主导。前嗅核的球内部分未显示任何免疫反应性结构。
