School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Photochem Photobiol. 2013 Mar-Apr;89(2):332-5. doi: 10.1111/j.1751-1097.2012.01243.x. Epub 2012 Oct 30.
A series of duplex DNA oligomers was prepared that contain a pyrene chromophore linked by a trimethylene chain (-(CH2 )3 -) to N(4) of a cytosine. The pyrene group stabilizes the DNA as evidenced by an increase in melting temperature. The absorption spectrum of the linked pyrene chromophore shows a temperature-dependent shift and there is also a strong induced circular dichroism spectrum attributed to the pyrene group. The fluorescence of the pyrene chromophore is strongly quenched at room temperature by linkage to the DNA, but it increases above the melting temperature. We attribute these observations to intramolecular intercalation of the pyrene group at a base pair adjacent to its linkage site at cytosine.
一系列的双链 DNA 寡聚体被制备，其中包含一个通过三亚甲基链（-(CH2 )3 -）连接到胞嘧啶的 N(4)的芘发色团。芘基团稳定 DNA，这一点可通过增加熔点来证明。连接的芘发色团的吸收光谱显示出与温度相关的位移，并且还有归因于芘基团的强烈诱导圆二色性光谱。芘发色团的荧光在室温下通过与 DNA 的连接而强烈猝灭，但在熔点以上增加。我们将这些观察归因于芘基团在与其连接位点相邻的碱基对处的分子内插入。
