1-ethynylpyrene-modified guanine and cytosine as optical labels for DNA hybridization.
作者信息
Wagner Clemens, Rist Manuela, Mayer-Enthart Elke, Wagenknecht Hans-Achim
机构信息
Technical University of Munich, Chemistry Department, Lichtenbergstr. 4, D-85747 Garching, Germany.
出版信息
Org Biomol Chem. 2005 Jun 7;3(11):2062-3. doi: 10.1039/b504079e. Epub 2005 May 4.
DOI:10.1039/b504079e
PMID:15917887
Abstract
1-ethynylpyrene shows remarkable absorption changes upon DNA hybridization when it is covalently attached to the 8-position of guanine. An absorption band at approximately 420 nm is only present in the duplex, exhibits thermal melting behaviour and provides the basis for a molecular beacon together with 1-ethynylpyrene-modified cytosine.
摘要
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