College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Mol Ecol. 2012 Oct;21(20):5073-87. doi: 10.1111/j.1365-294X.2012.05748.x. Epub 2012 Sep 18.
Previous studies have indicated that China is one of the domestication centres of Asian cultivated rice (Oryza sativa), and common wild rice (O. rufipogon) is the progenitor of O. sativa. However, the number of domestication times and the geographic origin of Asian cultivated rice in China are still under debate. In this study, 100 accessions of Asian cultivated rice and 111 accessions of common wild rice in China were selected to examine the relationship between O. sativa and O. rufipogon and thereby infer the domestication and evolution of O. sativa in China through sequence analyses of six gene regions, trnC-ycf6 in chloroplast genomes, cox3 in mitochondrial genomes and ITS, Ehd1, Waxy, Hd1 in nuclear genomes. The results indicated that the two subspecies of O. sativa (indica and japonica) were domesticated independently from different populations of O. rufipogon with gene flow occurring later from japonica to indica; Southern China was the genetic diversity centre of O. rufipogon, and the Pearl River basin near the Tropic of Cancer was the domestication centre of O. sativa in China.
先前的研究表明，中国是亚洲栽培稻（Oryza sativa）的驯化中心之一，普通野生稻（O. rufipogon）是 O. sativa 的祖先。然而，亚洲栽培稻在中国的驯化次数和地理起源仍存在争议。在这项研究中，选择了中国的 100 份亚洲栽培稻和 111 份普通野生稻，通过对叶绿体基因组中的 trnC-ycf6、线粒体基因组中的 cox3 以及核基因组中的 ITS、Ehd1、Waxy、Hd1 等六个基因区域的序列分析，考察了 O. sativa 和 O. rufipogon 之间的关系，从而推断了中国 O. sativa 的驯化和进化。结果表明，O. sativa 的两个亚种（籼稻和粳稻）是从不同的 O. rufipogon 群体独立驯化而来的，后来粳稻从 O. rufipogon 向籼稻发生基因流；中国南方是 O. rufipogon 的遗传多样性中心，而位于北回归线附近的珠江流域是中国 O. sativa 的驯化中心。
