PtdIns(4,5)P2 与 TRPM3 N 端的 CaM 结合域相互作用。
PtdIns(4,5)P2 interacts with CaM binding domains on TRPM3 N-terminus.
机构信息
Department of Protein Structures, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
出版信息
Channels (Austin). 2012 Nov-Dec;6(6):479-82. doi: 10.4161/chan.22177. Epub 2012 Sep 18.
Abstract
TRPM3 has been reported to play an important role in Ca(2+) homeostasis, but its gating mechanisms and regulation via Ca(2+) are unknown. Ca(2+) binding proteins such as calmodulin (CaM) could be probable modulators of this ion channel. We have shown that this protein binds to two independent domains, A35-K124 and H291-G382 on the TRPM3 N-terminus, which contain conserved hydrophobic as well as positively charged residues in specific positions, and that these residues have a crucial impact on its binding. We also showed that the other Ca(2+) binding protein, S100A1, is able to bind to these regions and that CaM and S100A1 compete for these binding sites on the TRPM3 N-terminus. Moreover, our results suggest that another very important TRP channel activity modulator, PtdIns(4,5)P(2), interacts with the CaM/S100A1 binding sites on the TRPM3 N-terminus with high affinity.
摘要
TRPM3 被报道在 Ca(2+) 稳态中发挥重要作用，但它的门控机制及其通过 Ca(2+)的调节尚不清楚。钙结合蛋白，如钙调蛋白 (CaM)，可能是这种离子通道的潜在调节剂。我们已经表明，这种蛋白结合到 TRPM3 N 端的两个独立结构域 A35-K124 和 H291-G382，这些结构域含有特定位置的保守疏水区和带正电荷的残基，这些残基对其结合有至关重要的影响。我们还表明，另一种 Ca(2+)结合蛋白 S100A1 能够结合到这些区域，并且 CaM 和 S100A1 竞争 TRPM3 N 端上的这些结合位点。此外，我们的结果表明，另一种非常重要的 TRP 通道活性调节剂，PtdIns(4,5)P(2)，以高亲和力与 TRPM3 N 端的 CaM/S100A1 结合位点相互作用。
相似文献
1
PtdIns(4,5)P2 interacts with CaM binding domains on TRPM3 N-terminus.PtdIns(4,5)P2 与 TRPM3 N 端的 CaM 结合域相互作用。
Channels (Austin). 2012 Nov-Dec;6(6):479-82. doi: 10.4161/chan.22177. Epub 2012 Sep 18.
2
Calmodulin and S100A1 protein interact with N terminus of TRPM3 channel.钙调蛋白和 S100A1 蛋白与 TRPM3 通道的 N 端相互作用。
J Biol Chem. 2012 May 11;287(20):16645-55. doi: 10.1074/jbc.M112.350686. Epub 2012 Mar 27.
3
Shared CaM- and S100A1-binding epitopes in the distal TRPM4 N terminus.TRPM4 远端 N 端的 Cam 和 S100A1 结合表位。
FEBS J. 2018 Feb;285(3):599-613. doi: 10.1111/febs.14362. Epub 2017 Dec 29.
4
Ca(2+) binding protein S100A1 competes with calmodulin and PIP2 for binding site on the C-terminus of the TPRV1 receptor.钙结合蛋白 S100A1 与钙调蛋白和 PIP2 竞争结合 TPRV1 受体 C 端结合位点。
ACS Chem Neurosci. 2015 Mar 18;6(3):386-92. doi: 10.1021/cn500250r. Epub 2014 Dec 31.
5
Mapping of CaM, S100A1 and PIP2-Binding Epitopes in the Intracellular N- and C-Termini of TRPM4.TRPM4 细胞内 N 端和 C 端钙调蛋白、S100A1 和 PIP2 结合表位的作图。
Int J Mol Sci. 2020 Jun 17;21(12):4323. doi: 10.3390/ijms21124323.
6
Ca-dependent regulation and binding of calmodulin to multiple sites of Transient Receptor Potential Melastatin 3 (TRPM3) ion channels.钙依赖性调节和钙调蛋白与瞬时受体电位 melastatin 3（TRPM3）离子通道多个位点的结合。
Cell Calcium. 2018 Jul;73:40-52. doi: 10.1016/j.ceca.2018.03.005. Epub 2018 Mar 31.
7
TRPM6 N-Terminal CaM- and S100A1-Binding Domains.TRPM6 N 端钙调蛋白和 S100A1 结合结构域。
Int J Mol Sci. 2019 Sep 9;20(18):4430. doi: 10.3390/ijms20184430.
8
The characterization of a novel S100A1 binding site in the N-terminus of TRPM1.TRPM1 N 端一个新型 S100A1 结合位点的特征描述。
Int J Biochem Cell Biol. 2016 Sep;78:186-193. doi: 10.1016/j.biocel.2016.07.014. Epub 2016 Jul 17.
9
TRPM5 Channel Binds Calcium-Binding Proteins Calmodulin and S100A1.瞬时受体电位阳离子通道蛋白5（TRPM5）通道结合钙结合蛋白钙调蛋白和S100A1。
Biochemistry. 2022 Mar 15;61(6):413-423. doi: 10.1021/acs.biochem.1c00647. Epub 2022 Feb 28.
10
Interaction of Calmodulin with TRPM: An Initiator of Channel Modulation.钙调蛋白与 TRPM 的相互作用：通道调节的启动子。
Int J Mol Sci. 2023 Oct 13;24(20):15162. doi: 10.3390/ijms242015162.
引用本文的文献
1
Phosphatidylinositol 4,5-bisphosphate activation mechanism of human KCNQ5.人源KCNQ5的磷脂酰肌醇4,5-二磷酸激活机制
Proc Natl Acad Sci U S A. 2025 Apr 8;122(14):e2416738122. doi: 10.1073/pnas.2416738122. Epub 2025 Apr 2.
2
Sex difference in TRPM3 channel functioning in nociceptive and vascular systems: an emerging target for migraine therapy in females?瞬时受体电位M3型（TRPM3）通道在伤害性感受和血管系统功能中的性别差异：女性偏头痛治疗的新靶点？
J Headache Pain. 2025 Feb 24;26(1):40. doi: 10.1186/s10194-025-01966-9.
3
Interaction of Calmodulin with TRPM: An Initiator of Channel Modulation.钙调蛋白与 TRPM 的相互作用：通道调节的启动子。
Int J Mol Sci. 2023 Oct 13;24(20):15162. doi: 10.3390/ijms242015162.
4
Regulation of ThermoTRP Channels by PIP2 and Cholesterol.PIP2 和胆固醇对热激 TRP 通道的调节。
Adv Exp Med Biol. 2023;1422:245-277. doi: 10.1007/978-3-031-21547-6_9.
5
Impaired TRPM3-dependent calcium influx and restoration using Naltrexone in natural killer cells of myalgic encephalomyelitis/chronic fatigue syndrome patients.肌痛性脑脊髓炎/慢性疲劳综合征患者自然杀伤细胞中 TRPM3 依赖性钙内流受损及纳曲酮的恢复作用。
J Transl Med. 2022 Feb 16;20(1):94. doi: 10.1186/s12967-022-03297-8.
6
The effect of IL-2 stimulation and treatment of TRPM3 on channel co-localisation with PIP and NK cell function in myalgic encephalomyelitis/chronic fatigue syndrome patients.白细胞介素-2 刺激和 TRPM3 治疗对肌痛性脑脊髓炎/慢性疲劳综合征患者中通道共定位与 NK 细胞功能的影响。
J Transl Med. 2021 Jul 15;19(1):306. doi: 10.1186/s12967-021-02974-4.
7
An Arrhythmic Mutation E7K Facilitates TRPM4 Channel Activation via Enhanced PIP Interaction.心律失常突变 E7K 通过增强 PIP 相互作用促进 TRPM4 通道激活。
Cells. 2021 Apr 22;10(5):983. doi: 10.3390/cells10050983.
8
TRPM3 in Brain (Patho)Physiology.大脑（病理）生理学中的瞬时受体电位阳离子通道亚家族M成员3
Front Cell Dev Biol. 2021 Feb 26;9:635659. doi: 10.3389/fcell.2021.635659. eCollection 2021.
9
Muscarinic Toxin 7 Signals Via Ca/Calmodulin-Dependent Protein Kinase Kinase β to Augment Mitochondrial Function and Prevent Neurodegeneration.毒蕈碱型乙酰胆碱受体毒素 7 通过钙/钙调蛋白依赖性蛋白激酶激酶β信号转导增强线粒体功能并预防神经退行性变。
Mol Neurobiol. 2020 Jun;57(6):2521-2538. doi: 10.1007/s12035-020-01900-x. Epub 2020 Mar 20.
10
TRPM3_miR-204: a complex locus for eye development and disease.TRPM3-miR-204：眼部发育和疾病的复杂基因座。
Hum Genomics. 2020 Feb 18;14(1):7. doi: 10.1186/s40246-020-00258-4.
本文引用的文献
1
TRPM3 is a nociceptor channel involved in the detection of noxious heat.瞬时受体电位通道蛋白 3 是一种伤害感受器通道，参与伤害性热的检测。
Neuron. 2011 May 12;70(3):482-94. doi: 10.1016/j.neuron.2011.02.051.
2
Characterization of calmodulin binding domains in TRPV2 and TRPV5 C-tails.钙调蛋白结合结构域在 TRPV2 和 TRPV5 C 端的特性研究。
Amino Acids. 2011 Feb;40(2):741-8. doi: 10.1007/s00726-010-0712-2. Epub 2010 Aug 5.
3
Unique S100 target protein interactions.独特的S100靶蛋白相互作用。
Gen Physiol Biophys. 2009;28 Spec No Focus:F39-46.
4
The interactions of the C-terminal region of the TRPC6 channel with calmodulin.TRPC6 通道 C 末端区域与钙调蛋白的相互作用。
Neurochem Int. 2010 Jan;56(2):363-6. doi: 10.1016/j.neuint.2009.11.009. Epub 2009 Nov 20.
5
Transient receptor potential channels meet phosphoinositides.瞬时受体电位通道与磷酸肌醇相遇。
EMBO J. 2008 Nov 5;27(21):2809-16. doi: 10.1038/emboj.2008.217. Epub 2008 Oct 16.
6
Ionic interactions are essential for TRPV1 C-terminus binding to calmodulin.离子相互作用对于TRPV1羧基末端与钙调蛋白的结合至关重要。
Biochem Biophys Res Commun. 2008 Oct 31;375(4):680-3. doi: 10.1016/j.bbrc.2008.08.094. Epub 2008 Aug 26.
7
Integration of phosphoinositide- and calmodulin-mediated regulation of TRPC6.磷酸肌醇和钙调蛋白介导的TRPC6调节的整合
Mol Cell. 2007 Feb 23;25(4):491-503. doi: 10.1016/j.molcel.2007.01.021.
8
Multiple roles of calmodulin and other Ca(2+)-binding proteins in the functional regulation of TRP channels.钙调蛋白及其他钙结合蛋白在瞬时受体电位通道功能调控中的多重作用
Pflugers Arch. 2005 Oct;451(1):105-15. doi: 10.1007/s00424-005-1427-1. Epub 2005 May 28.
9
Function and pharmacology of TRPM cation channels.瞬时受体电位M型（TRPM）阳离子通道的功能与药理学
Naunyn Schmiedebergs Arch Pharmacol. 2005 Apr;371(4):307-14. doi: 10.1007/s00210-005-1034-x.
10
A modular PIP2 binding site as a determinant of capsaicin receptor sensitivity.作为辣椒素受体敏感性决定因素的模块化磷脂酰肌醇-4,5-二磷酸（PIP2）结合位点
Science. 2003 May 23;300(5623):1284-8. doi: 10.1126/science.1083646.
Zotero 插件