Department of Chemistry, Mississippi State University, MS 39762, USA.
J Breath Res. 2012 Dec;6(4):041001. doi: 10.1088/1752-7155/6/4/041001. Epub 2012 Sep 18.
Here we describe an effective, reproducible, non-invasive volatile organic compound collection and analysis method for exhaled breath gas samples designed specifically for use with dogs. Conditions of the method were optimized, using a range of standard chemicals. This method utilizes a canine mask, two-way non-re-breathing valve, teflon connector, tubing and bag for sample collection. Collection is followed by condensation and headspace solid phase microextraction for sample concentration and gas chromatography-mass spectrometry for analysis. Custom-made glassware, designed to hold the SPME fiber assembly, was cooled to -10 °C and used for the collection of the condensate followed by 2 h of headspace extraction at 37 °C. Standards show LOD of 0.6-16.8 ppbv, LOQ between 2.1-55.8 ppbv, and good linearity with R(2) between 0.996-0.999 (RSD% 10-19). The method was verified with preliminary results from three dogs demonstrating that this technique is capable of collecting, identifying and quantifying volatile organic chemical constituents in different breath samples.
在这里，我们描述了一种针对犬只设计的有效、可重复、非侵入性的呼出气样本挥发性有机化合物采集和分析方法。我们使用一系列标准化学品对该方法的条件进行了优化。该方法使用犬用口罩、双向非再呼吸阀、特氟龙接头、管和袋子进行样本采集。采集后，通过冷凝和顶空固相微萃取进行样品浓缩，然后进行气相色谱-质谱分析。定制的玻璃器皿用于容纳 SPME 纤维组件，将其冷却至-10°C，用于收集冷凝物，然后在 37°C 下进行 2 小时的顶空萃取。标准显示检测限为 0.6-16.8 ppbv，定量限为 2.1-55.8 ppbv，线性良好，相关系数为 0.996-0.999（RSD%为 10-19）。该方法通过对三只狗的初步结果进行了验证，证明了该技术能够采集、识别和定量不同呼吸样本中的挥发性有机化学成分。
