Ghiţă C, Vîlcea I D, Dumitrescu M, Vîlcea Alina Maria, Mirea C S, Aşchie Mariana, Vasilescu Florina
Dr Alexandru Gafencu Emergency Military Hospital, Constanta, Romania.
Rom J Morphol Embryol. 2012;53(3):549-56.
This study aim was to identify the relations between tumor suppressor genes (p53, bcl-2, PTEN), nuclear proliferative antigen Ki-67 and epidemiologic, morphologic and histologic patient related factors, in colorectal cancer.
Twenty-two prospectively collected colorectal cancer resection specimens were histologically prepared, using standard paraffin-embedded and Hematoxylin-Eosin staining method; for immunohistochemical study, the Streptavidin-Biotin (sABC)/Horseradish Peroxidase (HRP) method was used.
P53 was positive in 86.36% of cases, more intense (>50%) in rectal cancer and in women 59.16 ± 9.49-year-old; the G1/2 adenocarcinoma was dominant. Bcl-2 was positive in 18.18% of the cases, in distal colorectal cancer, only in men, 63.5 ± 13.2-year-old. PTEN was positive in 95.45% of the cases; intense positivity was recorded in 12 men and six women, 61.47 ± 11.67-year-old, in rectal topography. Ki-67 was positive in 86.36% of the cases, more intense in four men and four women, 63.45 ± 12.22-year-old, in proximal and advanced colorectal cancers (pT3N1/2 - 62.5%).
Tumor suppressor genes mutations are often present in colorectal cancer; the intensity of the expression of these mutations varies, which could explain the different prognosis for these patients.
本研究旨在确定结直肠癌中肿瘤抑制基因（p53、bcl-2、PTEN）、核增殖抗原Ki-67与患者的流行病学、形态学和组织学相关因素之间的关系。
前瞻性收集22例结直肠癌切除标本，采用标准石蜡包埋和苏木精-伊红染色法进行组织学制备；免疫组织化学研究采用链霉亲和素-生物素（sABC）/辣根过氧化物酶（HRP）法。
p53在86.36%的病例中呈阳性，在直肠癌和59.16±9.49岁的女性中表达更强（>50%）；G1/2腺癌占主导。bcl-2在18.18%的病例中呈阳性，仅在远端结直肠癌、63.5±13.2岁的男性中出现。PTEN在95.45%的病例中呈阳性；在直肠部位，12名男性和6名女性（61.47±11.67岁）中记录到强阳性。Ki-67在86.36%的病例中呈阳性，在近端和进展期结直肠癌（pT3N1/2 - 62.5%）中，4名男性和4名女性（63.45±12.22岁）中表达更强。
肿瘤抑制基因突变在结直肠癌中经常出现；这些突变的表达强度各不相同，这可能解释了这些患者不同的预后情况。
