Yun Fen, Jia Yongfeng, Li Xiuxia, Yuan Li, Sun Qinnuan, Yu Huiling, Shi Lin, Yuan Hongwei
Department of Pathology, The First Affiliated Hospital of Inner Mongolia Medical University Huhhot, 010059, China.
Int J Clin Exp Pathol. 2013 Sep 15;6(10):2112-20. eCollection 2013.
A high frequency of mutations at the PTEN locus has been noticed in carcinoma of lung. However, the role of PTEN alternations and its association with outcome variables in the genesis of lung carcinoma are not understood fully. The purpose of our study was to examine the impact of EGFR, TGF-α, P-AKT and PTEN in the genesis of non-small cell lung cancer (NSCLC). Total numbers of 66 histopathologically confirmed cases of NSCLC and 10 cases of benign control samples embedded with wax were studied. We assessed EGFR, TGF-α and P-AKT by the use of specific antibody through immunohistochemistry as directed by the manufacturer, and detected PTEN expression by in situ hybridization. There were progressive loss of PTEN expression and significant increasing in EGFR, TGF-α, P-AKT expression from benign samples to NSCLC (p<0.05). The overexpression of EGFR, TGF-α, P-AKT and loss of PTEN expression were correlated to differentiation extent of cancer tissue, metastasis of lymph nodes and histological classification. Thus, alteration of EGFR, TGF-α, P-AKT and PTEN are likely important molecular events in pathogenesis and carcinogenesis of NSCLC.
在肺癌中已注意到PTEN基因座的高频突变。然而，PTEN改变在肺癌发生中的作用及其与预后变量的关联尚未完全明了。我们研究的目的是检测表皮生长因子受体（EGFR）、转化生长因子-α（TGF-α）、磷酸化蛋白激酶B（P-AKT）和PTEN在非小细胞肺癌（NSCLC）发生中的影响。研究了66例经组织病理学确诊的NSCLC病例以及10例石蜡包埋的良性对照样本。按照制造商的说明，我们通过免疫组织化学使用特异性抗体评估EGFR、TGF-α和P-AKT，并通过原位杂交检测PTEN表达。从良性样本到NSCLC，PTEN表达逐渐丧失，EGFR、TGF-α、P-AKT表达显著增加（p<0.05）。EGFR、TGF-α、P-AKT的过表达以及PTEN表达的丧失与癌组织的分化程度、淋巴结转移和组织学分类相关。因此，EGFR、TGF-α、P-AKT和PTEN的改变可能是非小细胞肺癌发病机制和癌变过程中的重要分子事件。
