Rug Melanie, Maier Alexander G
Research School of Biology, The Australian National University, Canberra, ACT, Australia.
Methods Mol Biol. 2013;923:75-98. doi: 10.1007/978-1-62703-026-7_6.
Genetic manipulation of Plasmodium falciparum remains very challenging, mainly due to the parasite genome's high A/T-richness and low transfection efficiency. This chapter includes methods for generating transient and stable transfections by electroporation, allelic replacement with tagged genes, gene deletion, and the analysis of all the above.
恶性疟原虫的基因操作仍然极具挑战性，主要原因是该寄生虫基因组富含A/T且转染效率低。本章介绍了通过电穿孔进行瞬时和稳定转染、用标记基因进行等位基因替换、基因缺失以及上述所有操作的分析方法。
